DC Field | Value | Language |
---|---|---|
dc.contributor.author | C H Kang | - |
dc.contributor.author | B C Moon | - |
dc.contributor.author | H C Park | - |
dc.contributor.author | S C Koo | - |
dc.contributor.author | J M Jeon | - |
dc.contributor.author | Y H Cheong | - |
dc.contributor.author | W S Chung | - |
dc.contributor.author | C O Lim | - |
dc.contributor.author | J Y Kim | - |
dc.contributor.author | Byung Dae Yoon | - |
dc.contributor.author | S Y Lee | - |
dc.contributor.author | Cha Young Kim | - |
dc.date.accessioned | 2017-04-19T09:27:23Z | - |
dc.date.available | 2017-04-19T09:27:23Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.uri | 10.1016/j.bbagen.2011.06.021 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10472 | - |
dc.description.abstract | Background: The C2 domain is a Ca2+/phospholipid-binding motif found in many proteins involved in signal transduction or membrane trafficking. OsERG3 is a homolog of OsERG1, a gene encoding a small C2-domain protein in rice. Methods: OsERG3 Ca2+-binding and phospholipid-binding assays were carried out using 3H-labeled phospholipid liposomes and a 45Ca2+ overlay assay, respectively. Cytosolic expression of OsERG3 was investigated by Western blot analysis and the OsERG3::smGFP transient expression assay. Results: OsERG3 transcript levels were greatly enhanced by treatment with a fungal elicitor and Ca2+-ionophore. OsERG3 protein proved unable to interact with phospholipids regardless of the presence or absence of Ca2+ ions. Nonetheless, OsERG3 displayed calcium-binding activity in an in vitro45Ca2+-binding assay, a property not observed with OsERG1. The cytosolic location of OsERG3 was not altered by the presence of fungal elicitor or Ca2+-ionophore. Conclusions: OsERG3 encodes a small C2-domain protein consisting of a single C2 domain. OsERG3 binds Ca2+ ions but not phospholipids. OsERG3 is a cytosolic soluble protein. The OsERG3 gene may play a role in signaling pathway involving Ca2+ ions. General significance: The data demonstrate that OsERG3 is an unusual small C2-domain protein containing a Ca2+- binding module but lacking phospholipid-binding properties. | - |
dc.publisher | Elsevier | - |
dc.title | Rice OsERG3 encodes an unusual small C2-domain protein containing a Ca2+-binding module but lacking phospholipid-binding properties | - |
dc.title.alternative | Rice OsERG3 encodes an unusual small C2-domain protein containing a Ca2+-binding module but lacking phospholipid-binding properties | - |
dc.type | Article | - |
dc.citation.title | Biochimica et Biophysica Acta-General Subjects | - |
dc.citation.number | 12 | - |
dc.citation.endPage | 1322 | - |
dc.citation.startPage | 1317 | - |
dc.citation.volume | 1810 | - |
dc.contributor.affiliatedAuthor | Byung Dae Yoon | - |
dc.contributor.affiliatedAuthor | Cha Young Kim | - |
dc.contributor.alternativeName | 강창호 | - |
dc.contributor.alternativeName | 문병철 | - |
dc.contributor.alternativeName | 박형철 | - |
dc.contributor.alternativeName | 구성철 | - |
dc.contributor.alternativeName | 전주미 | - |
dc.contributor.alternativeName | 정용화 | - |
dc.contributor.alternativeName | 정우식 | - |
dc.contributor.alternativeName | 임채오 | - |
dc.contributor.alternativeName | 김재연 | - |
dc.contributor.alternativeName | 윤병대 | - |
dc.contributor.alternativeName | 이상열 | - |
dc.contributor.alternativeName | 김차영 | - |
dc.identifier.bibliographicCitation | Biochimica et Biophysica Acta-General Subjects, vol. 1810, no. 12, pp. 1317-1322 | - |
dc.identifier.doi | 10.1016/j.bbagen.2011.06.021 | - |
dc.subject.keyword | Ca2+/ phospholipid binding | - |
dc.subject.keyword | Rice | - |
dc.subject.keyword | Signal transduction | - |
dc.subject.keyword | Small C2-domain protein | - |
dc.subject.local | Ca2+/ phospholipid binding | - |
dc.subject.local | Ca2+/phospholipid-binding | - |
dc.subject.local | Rice | - |
dc.subject.local | Rice (Oryza sativa L) | - |
dc.subject.local | Rice (Oryza sativa L.) | - |
dc.subject.local | rice | - |
dc.subject.local | rice (Oryza sativa) | - |
dc.subject.local | Oryza sativa | - |
dc.subject.local | Oryza sativa L | - |
dc.subject.local | Oryza sativa L. | - |
dc.subject.local | oryza sativa | - |
dc.subject.local | signal transduction | - |
dc.subject.local | Signal transduction | - |
dc.subject.local | Signal trasduction | - |
dc.subject.local | Small C2-domain protein | - |
dc.subject.local | small C2-domain protein | - |
dc.description.journalClass | Y | - |
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