Functional pentameric formation via coexpression of the Escher coli heat-labile enterotoxin B subunit and its fusion protein subunit with a neutralizing epitope of ApxIIA exotoxin improves the mucosal immugenicity = 기능적 오합체 형성에 의한 면역력 강화

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dc.contributor.authorJ M Kim-
dc.contributor.authorS M Park-
dc.contributor.authorJ A Kim-
dc.contributor.authorJ A Park-
dc.contributor.authorM H Yi-
dc.contributor.authorN S Kim-
dc.contributor.authorJ L Bae-
dc.contributor.authorSung Goo Park-
dc.contributor.authorY S Jang-
dc.contributor.authorM S Yang-
dc.contributor.authorD H Kim-
dc.date.accessioned2017-04-19T09:27:23Z-
dc.date.available2017-04-19T09:27:23Z-
dc.date.issued2011-
dc.identifier.issn1556-6811-
dc.identifier.uri10.1128/CVI.05230-11ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10474-
dc.description.abstractA coexpression strategy in Saccharomyces cerevisiae using episomal and integrative vectors for the Escherichia coli heat-labile enterotoxin B subunit (LTB) and a fusion protein of an ApxIIA toxin epitope produced by Actinobacillus pleuropneumoniae coupled to LTB, respectively, was adapted for the hetero-oligomerization of LTB and the LTB fusion construct. Enzyme-linked immunosorbent assay (ELISA) with GM1 ganglioside indicated that the LTB fusion construct, along with LTB, was oligomerized to make the functional heteropentameric form, which can bind to receptors on the mucosal epithelium. The antigen-specific antibody titer of mice orally administered antigen was increased when using recombinant yeast coexpressing the pentameric form instead of recombinant yeast expressing either the LTB fusion form or antigen alone. Better protection against challenge infection with A. pleuropneumoniae was also observed for coexpression in recombinant yeast compared with others. The present study clearly indicated that the coexpression strategy enabled the LTB fusion construct to participate in the pentameric formation, resulting in an improved induction of systemic and mucosal immune responses.-
dc.publisherAmer Soc Microbiology-
dc.titleFunctional pentameric formation via coexpression of the Escher coli heat-labile enterotoxin B subunit and its fusion protein subunit with a neutralizing epitope of ApxIIA exotoxin improves the mucosal immugenicity = 기능적 오합체 형성에 의한 면역력 강화-
dc.title.alternativeFunctional pentameric formation via coexpression of the Escher coli heat-labile enterotoxin B subunit and its fusion protein subunit with a neutralizing epitope of ApxIIA exotoxin improves the mucosal immugenicity and protection against challenge by Actin-
dc.typeArticle-
dc.citation.titleClinical and Vaccine Immunology-
dc.citation.number12-
dc.citation.endPage2177-
dc.citation.startPage2168-
dc.citation.volume18-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.alternativeName김정미-
dc.contributor.alternativeName박승문-
dc.contributor.alternativeName김정애-
dc.contributor.alternativeName박진아-
dc.contributor.alternativeName이민희-
dc.contributor.alternativeName김난순-
dc.contributor.alternativeName배종례-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName장용석-
dc.contributor.alternativeName양문식-
dc.contributor.alternativeName김대혁-
dc.identifier.bibliographicCitationClinical and Vaccine Immunology, vol. 18, no. 12, pp. 2168-2177-
dc.identifier.doi10.1128/CVI.05230-11-
dc.description.journalClassY-
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Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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