Preparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography

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dc.contributor.authorG H Quan-
dc.contributor.authorSei Ryang Oh-
dc.contributor.authorHyuk Hwan Song-
dc.contributor.authorHyeong Kyu Lee-
dc.contributor.authorY W Chin-
dc.date.accessioned2017-04-19T09:27:27Z-
dc.date.available2017-04-19T09:27:27Z-
dc.date.issued2011-
dc.identifier.issnI000-0171-
dc.identifier.uri10.3839/jksabc.2011.094ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10480-
dc.description.abstractA simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePreparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography-
dc.title.alternativePreparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography-
dc.typeArticle-
dc.citation.titleJournal of Korean Society for Applied Biological Chemistry-
dc.citation.number4-
dc.citation.endPage627-
dc.citation.startPage623-
dc.citation.volume54-
dc.contributor.affiliatedAuthorSei Ryang Oh-
dc.contributor.affiliatedAuthorHyuk Hwan Song-
dc.contributor.affiliatedAuthorHyeong Kyu Lee-
dc.contributor.alternativeNameQuan-
dc.contributor.alternativeName오세량-
dc.contributor.alternativeName송혁환-
dc.contributor.alternativeName이형규-
dc.contributor.alternativeName진영원-
dc.identifier.bibliographicCitationJournal of Korean Society for Applied Biological Chemistry, vol. 54, no. 4, pp. 623-627-
dc.identifier.doi10.3839/jksabc.2011.094-
dc.subject.keyword8-methoxypsoralen-
dc.subject.keywordHigh-speed counter-current chromatography-
dc.subject.keywordPulsatilla chinensis-
dc.subject.local8-methoxypsoralen-
dc.subject.local8-Methoxypsoralen-
dc.subject.localHigh-speed counter-current chromatography-
dc.subject.localhigh-speed counter-current chromatography (HSCCC)-
dc.subject.localPulsatilla chinensis-
dc.description.journalClassN-
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