Fermentation strategies for 1,3-propanediol production from glycerol using a genetically engineered Klebsiella pneumoniae strain to eliminate by-product formation

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Title
Fermentation strategies for 1,3-propanediol production from glycerol using a genetically engineered Klebsiella pneumoniae strain to eliminate by-product formation
Author(s)
Baek Rock OhJeong-Woo SeoSun-Yeon Heo; Won Kyung Hong; L H Luo; J H Son; D H Park; Chul Ho Kim
Bibliographic Citation
Bioprocess and Biosystems Engineering, vol. 35, no. 1, pp. 159-165
Publication Year
2012
Abstract
We generated a genetically engineered Klebsiella pneumoniae strain (AK-VOT) to eliminate by-product formation during production of 1,3-propanediol (1,3-PD) from glycerol. In the present study, the glycerol-metabolizing properties of the recombinant strain were examined during fermentation in a 5 L bioreactor. As expected, by-product formation was completely absent (except for acetate) when the AK-VOT strain fermented glycerol. However, 1,3-PD productivity was severely reduced owing to a delay in cell growth attributable to a low rate of glycerol consumption. This problem was solved by establishing a two-stage process separating cell growth from 1,3-PD production. In addition, nutrient co-supplementation, especially with starch, significantly increased 1,3-PD production from glycerol during fed-batch fermentation by AK-VOT in the absence of by-product formation.
Keyword
1,3-PropanediolBy-product formationGlycerolKlebsiella pneumoniaeNutrient co-supplementationTwo-stage fermentation
ISSN
1615-7591
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00449-011-0603-2
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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