Optimization of culture conditions for 1,3-propanediol production from glycerol using a mutant strain of Klebsiella pneumoniae

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dc.contributor.authorBaek Rock Oh-
dc.contributor.authorJeong-Woo Seo-
dc.contributor.authorSun-Yeon Heo-
dc.contributor.authorWon Kyung Hong-
dc.contributor.authorL H Luo-
dc.contributor.authorSeonghun Kim-
dc.contributor.authorD H Park-
dc.contributor.authorChul Ho Kim-
dc.date.accessioned2017-04-19T09:27:32Z-
dc.date.available2017-04-19T09:27:32Z-
dc.date.issued2012-
dc.identifier.issn0273-2289-
dc.identifier.uri10.1007/s12010-011-9409-6ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10497-
dc.description.abstractIn the present work, mutant strains of Klebsiella pneumoniae with deletions of the als gene encoding acetolactate synthase involved in synthesis of 2,3-butanediol, the ldhA gene encoding lactate dehydrogenase required for lactate synthesis, or both genes, were prepared. Production of 1,3-propanediol (1,3-PD) from glycerol was enhanced in the ldhA mutant strain (ΔldhA), but lower in Δals or Δals ΔldhA mutant strains compared to the parent strain, concomitant with a reduction in the glycerol consumption rate, indicating that deletion of ldhA alone was useful to improve 1,3-PD production. Fed-batch fermentation analysis revealed that, in the ΔldhA mutant strain, 1,3-PD production was higher at low pH than at neutral pH; the reverse was true for the parent strain. Further optimization of culture conditions, by variation of aeration and glycerol feed rates, dramatically improved the production of 1,3-PD by the mutant strain. The maximum level attained was 102.7 g l-1 of 1,3-PD from glycerol.-
dc.publisherSpringer-
dc.titleOptimization of culture conditions for 1,3-propanediol production from glycerol using a mutant strain of Klebsiella pneumoniae-
dc.title.alternativeOptimization of culture conditions for 1,3-propanediol production from glycerol using a mutant strain of Klebsiella pneumoniae-
dc.typeArticle-
dc.citation.titleApplied Biochemistry and Biotechnology-
dc.citation.number1-
dc.citation.endPage137-
dc.citation.startPage127-
dc.citation.volume166-
dc.contributor.affiliatedAuthorBaek Rock Oh-
dc.contributor.affiliatedAuthorJeong-Woo Seo-
dc.contributor.affiliatedAuthorSun-Yeon Heo-
dc.contributor.affiliatedAuthorWon Kyung Hong-
dc.contributor.affiliatedAuthorSeonghun Kim-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.alternativeName오백록-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName허선연-
dc.contributor.alternativeName홍원경-
dc.contributor.alternativeNameLuo-
dc.contributor.alternativeName김성훈-
dc.contributor.alternativeName박돈희-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationApplied Biochemistry and Biotechnology, vol. 166, no. 1, pp. 127-137-
dc.identifier.doi10.1007/s12010-011-9409-6-
dc.subject.keyword1,3-Propanediol-
dc.subject.keywordGene deletion-
dc.subject.keywordGlycerol-
dc.subject.keywordKlebsiella pneumoniae-
dc.subject.keywordOptimized culture conditions-
dc.subject.local1,3-propanediol-
dc.subject.local1,3-Propanediol-
dc.subject.localgene-deletion-
dc.subject.localGene deletion-
dc.subject.localgene deletion-
dc.subject.localGlycerol-
dc.subject.localglycerol-
dc.subject.localKlebsiella pneumonia-
dc.subject.localKlebsiella pneumoniae-
dc.subject.localOptimized culture conditions-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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