Abnomalous somatic embryos formation and plant regeneration from the cultures of immature embryos of Camellia japonica L. = 동백나무 미숙배 배양으로부터 비정상 체세포배 형성과 식물체 재생

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Title
Abnomalous somatic embryos formation and plant regeneration from the cultures of immature embryos of Camellia japonica L. = 동백나무 미숙배 배양으로부터 비정상 체세포배 형성과 식물체 재생
Author(s)
J H Choi; Suk Yoon Kwon; P S Choi
Bibliographic Citation
Journal of Plant Biotechnology, vol. 38, no. 4, pp. 258-262
Publication Year
2011
Abstract
Embryogenic callus was induced from the cultures of immature embryos of Camellia japonica L. on Murashige & Skoog’s (MS) solid medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D), and then the embryogenic callus was proliferated on same medium for 4 weeks over. The embryogenic callus was sub-cultured on MS basal medium without 2,4-D to produce coyledonary stage of somatic embryo. The frequency (%) of somatic embryogenesis was 25.1%, and the majority of somatic embryos formed had a abnormal morphology with cupshaped cotyledon (48.3%), one cotyledon (12.6%), three cotyledons (9.4%), four cotyledons (1.9%), whereas was only normal morphology with two cotyledon (27.5%). When the somatic embryos with normal or abnormal cotyledons transfer to MS basal medium or ½ MS medium with/or without plant growth regulators (GA3, IBA) for regeneration, the frequency (%) of two-cotyledon embryos regenerated into plantlets was higher 11.1% than one cotyledon (0.0~8.3 %), three cotyledons (0.0~5.8%), four cotyledons (0.0%), cup-shaped (0.3~4.2%). These results demonstrated that the anomalous cotyledons of somatic embryos could caused to decrease the rate of plant regeneration.
Keyword
Abnormal cotyledonplant regenerationsomatic embryos
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2011.38.4.258
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
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