Tenacibaculum geojense sp. nov., isolated from seawater

Abstract

A Gram-negative, non-flagellated, non-spore-forming bacterium, designated YCS-6 T, that was motile by gliding, was isolated from seawater on the southern coast of Korea. Strain YCS-6 T grew optimally at 30 °C and with 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YCS-6 T fell within the genus Tenacibaculum and was most closely associated with Tenacibaculum litopenaei B-I T, with which the isolate exhibited 95.9% 16S rRNA gene sequence similarity. Sequence similarity between strain YCS-6 T and other members of the genus Tenacibaculum was 93.8-95.7 %. Strain YCS-6 Tcontained menaquinone-6 (MK-6) as the predominant respiratory quinone and iso-C 15: 0, summed feature 3 (iso-C 15: 0 2-OH and/or C 16: 1ω7c), iso-C 15: 0 3-OH and iso-C 15: 1 G as the major fatty acids. The DNA G+C content was 32.7 mol%. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain YCS-6 T from all other members of the genus Tenacibaculum. On the basis of our phenotypic, chemotaxonomic and phylogenetic data, strain YCS-6 T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum geojense sp. nov. is proposed. The type strain is YCS-6 T (=KCTC 23423 T =CCUG 60527 T).