Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim

Abstract

A Gram-negative-staining, non-motile rod, designated GG-w14 T, was isolated from the rhizosphere of Angelica polymorpha Maxim. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolate belonged to the genus Mucilaginibacter and exhibited 93.9-97.4% 16S rRNA gene sequence similarity with recognized members of the genus Mucilaginibacter (closest relative Mucilaginibacter gossypii Gh-67 T). DNA-DNA relatedness between strain GG-w14 T and M. gossypii KCTC 22380 T was,41 %. Strain GG-w14 T grew at 4-35 6C, at pH 5.0-8.0 and with 0-1% (w/v) NaCl. The isolate hydrolysed casein, CM-cellulose and starch and contained menaquinone 7 as the major menaquinone. The major cellular fatty acids were summed feature 3 ( C16:1v7c and/or iso-C15: 0 2-OH; 39.9 %), iso-C15: 0 (24.2 %) and iso-C17: 0 3-OH (12.4 %). The DNA G+C content was 42.5 mol%. These data suggest that strain GG-w14 T should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter angelicae sp. nov. is proposed. The type strain is GG-w14 T (=KCTC 23250 T=NCAIM B 0241 5T).