Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe)

Abstract

A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62 T, was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62 T grew at 15-30 °C and pH 5.0-8.5. The isoprenoid quinones were menaquinones MK-11 (77.1 %), MK-10 (11.7 %) and MK-12 (11.2 %). The major cellular fatty acids were anteiso-C 15:0 (34.6 %), anteiso-C 17:0 (29.8 %), iso-C 16:0 (17.0 %) and cyclohexyl-C 17:0 (11.4 %). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62 T was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62 T was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11 T (98.08% similarity). The level of DNA-DNA relatedness between strain RB-62 T and H. ginsengi wged11 T was 43.2% (reciprocal 66.7 %). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62T from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62 T is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62 T (=KCTC 19653 T=JCM 16117 T).