Dynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR

Cited 41 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorA Srivastava-
dc.contributor.authorGang Guk Choi-
dc.contributor.authorChi-Yong Ahn-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorA K Ravi-
dc.contributor.authorR K Asthana-
dc.date.accessioned2017-04-19T09:27:59Z-
dc.date.available2017-04-19T09:27:59Z-
dc.date.issued2012-
dc.identifier.issn00431354-
dc.identifier.uri10.1016/j.watres.2011.11.056ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10524-
dc.description.abstractCyanobacterial blooms in eutrophied water body are generally composed of various genotypes with or without microcystin-producing genes (mcy gene cluster). Thus there is a need for quantification of potent toxin producing strains. The present study aimed at identifying microcystin variants and its producer strains in Durgakund pond, Varanasi, India, based on quantification of cpcBA-IGS and mcyA (condensation domain) genes using real-time PCR and LC-MS. Increase in microcystin concentrations was correlated with increase in mcyA copy number and the level of pigments (chlorophyll a, phycocyanin and carotenoids). Also, selected environmental factors (water temperature, light irradiance, rainfall, pH, N and P) and the concentration of microcystin variants (MC-LR, -RR and -YR) were also assessed in samples during May 2010 to April 2011 to establish the possible correlation among these parameters. Nutrients favored cyanobacterial bloom but it could not be correlated with the levels of microcystin variants and seemed to be geographically specific. Microcystis sp. dominant in the pond comprised potentially toxigenic cells. The ratio of potentially toxigenic Microcystis sp. to that of total Microcystis sp. ranged from 0% to 14%. Such studies paved the way to identify and quantify the most potent microcystin producer in the tropical aquatic body.-
dc.publisherElsevier-
dc.titleDynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR-
dc.title.alternativeDynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR-
dc.typeArticle-
dc.citation.titleWater Research-
dc.citation.number3-
dc.citation.endPage827-
dc.citation.startPage817-
dc.citation.volume46-
dc.contributor.affiliatedAuthorChi-Yong Ahn-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.alternativeNameSrivastava-
dc.contributor.alternativeName최강국-
dc.contributor.alternativeName안치용-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeNameRavi-
dc.contributor.alternativeNameAsthana-
dc.identifier.bibliographicCitationWater Research, vol. 46, no. 3, pp. 817-827-
dc.identifier.doi10.1016/j.watres.2011.11.056-
dc.subject.keywordLC-MS-
dc.subject.keywordMicrocystin-
dc.subject.keywordMicrocystis sp.-
dc.subject.keywordReal-time PCR-
dc.subject.localLC-MS-
dc.subject.localMicrocystin-
dc.subject.localMicrocystis sp.-
dc.subject.localReal-time PCR-
dc.subject.localReal time PCR-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.