Structural and fuctional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii = Pichia ciferrii의 S-adenosylmethionine (SAM) synthetase에 대한 구조 및 기능적 특성

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dc.contributor.authorS Yoon-
dc.contributor.authorW Lee-
dc.contributor.authorMin-Soo Kim-
dc.contributor.authorT D Kim-
dc.contributor.authorY Ryu-
dc.date.accessioned2017-04-19T09:28:41Z-
dc.date.available2017-04-19T09:28:41Z-
dc.date.issued2012-
dc.identifier.issn1615-7591-
dc.identifier.uri10.1007/s00449-011-0640-xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10557-
dc.description.abstractS-adenosylmethionine synthetase (SAM-s) catalyzes the synthesis of S-adenosylmethionine (SAM), which is essential for methylation, transcription, proliferation, and production of secondary metabolites. Here SAM-s from Pichia ciferrii were selectively cloned using RNA CapFishing and rapid amplification of cDNA ends (RACE). The putative full-length cDNA of SAM-s encoded a 383 amino acid protein (42.6 kDa), which has highly conserved metal binding sites, a phosphate-binding site, and functionally important motifs. The corresponding enzyme was over-expressed in a heterologous host of Pichia pastoris, and then purified to a homogenous form. Enzyme kinetics, immunoblotting, circular dichroism (CD), high performance liquid chromatography (HPLC), and molecular modeling were conducted to characterize the SAM-s from P. ciferrii. Structural and functional studies of SAM-s will provide important insights for industrial applications.-
dc.publisherSpringer-
dc.titleStructural and fuctional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii = Pichia ciferrii의 S-adenosylmethionine (SAM) synthetase에 대한 구조 및 기능적 특성-
dc.title.alternativeStructural and fuctional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii-
dc.typeArticle-
dc.citation.titleBioprocess and Biosystems Engineering-
dc.citation.number1-
dc.citation.endPage181-
dc.citation.startPage173-
dc.citation.volume35-
dc.contributor.affiliatedAuthorMin-Soo Kim-
dc.contributor.alternativeName윤상영-
dc.contributor.alternativeName이원규-
dc.contributor.alternativeName김민수-
dc.contributor.alternativeName김도만-
dc.contributor.alternativeName유연우-
dc.identifier.bibliographicCitationBioprocess and Biosystems Engineering, vol. 35, no. 1, pp. 173-181-
dc.identifier.doi10.1007/s00449-011-0640-x-
dc.subject.keywordS-adenosylmethionine-
dc.subject.keywordSAM synthetase-
dc.subject.keywordPichia ciferrii-
dc.subject.keywordRNA CapFishing-
dc.subject.keywordRapid amplification of cDNA ends-
dc.subject.localS-adenosylmethionine-
dc.subject.localS-Adenosylmethionine-
dc.subject.localSAM synthetase-
dc.subject.localPichia ciferrii-
dc.subject.localRNA CapFishing-
dc.subject.localRapid amplification of cDNA ends-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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