DC Field | Value | Language |
---|---|---|
dc.contributor.author | S Yoon | - |
dc.contributor.author | W Lee | - |
dc.contributor.author | Min-Soo Kim | - |
dc.contributor.author | T D Kim | - |
dc.contributor.author | Y Ryu | - |
dc.date.accessioned | 2017-04-19T09:28:41Z | - |
dc.date.available | 2017-04-19T09:28:41Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | 10.1007/s00449-011-0640-x | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10557 | - |
dc.description.abstract | S-adenosylmethionine synthetase (SAM-s) catalyzes the synthesis of S-adenosylmethionine (SAM), which is essential for methylation, transcription, proliferation, and production of secondary metabolites. Here SAM-s from Pichia ciferrii were selectively cloned using RNA CapFishing and rapid amplification of cDNA ends (RACE). The putative full-length cDNA of SAM-s encoded a 383 amino acid protein (42.6 kDa), which has highly conserved metal binding sites, a phosphate-binding site, and functionally important motifs. The corresponding enzyme was over-expressed in a heterologous host of Pichia pastoris, and then purified to a homogenous form. Enzyme kinetics, immunoblotting, circular dichroism (CD), high performance liquid chromatography (HPLC), and molecular modeling were conducted to characterize the SAM-s from P. ciferrii. Structural and functional studies of SAM-s will provide important insights for industrial applications. | - |
dc.publisher | Springer | - |
dc.title | Structural and fuctional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii = Pichia ciferrii의 S-adenosylmethionine (SAM) synthetase에 대한 구조 및 기능적 특성 | - |
dc.title.alternative | Structural and fuctional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii | - |
dc.type | Article | - |
dc.citation.title | Bioprocess and Biosystems Engineering | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 181 | - |
dc.citation.startPage | 173 | - |
dc.citation.volume | 35 | - |
dc.contributor.affiliatedAuthor | Min-Soo Kim | - |
dc.contributor.alternativeName | 윤상영 | - |
dc.contributor.alternativeName | 이원규 | - |
dc.contributor.alternativeName | 김민수 | - |
dc.contributor.alternativeName | 김도만 | - |
dc.contributor.alternativeName | 유연우 | - |
dc.identifier.bibliographicCitation | Bioprocess and Biosystems Engineering, vol. 35, no. 1, pp. 173-181 | - |
dc.identifier.doi | 10.1007/s00449-011-0640-x | - |
dc.subject.keyword | S-adenosylmethionine | - |
dc.subject.keyword | SAM synthetase | - |
dc.subject.keyword | Pichia ciferrii | - |
dc.subject.keyword | RNA CapFishing | - |
dc.subject.keyword | Rapid amplification of cDNA ends | - |
dc.subject.local | S-adenosylmethionine | - |
dc.subject.local | S-Adenosylmethionine | - |
dc.subject.local | SAM synthetase | - |
dc.subject.local | Pichia ciferrii | - |
dc.subject.local | RNA CapFishing | - |
dc.subject.local | Rapid amplification of cDNA ends | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.