Monitoring of adipogenic differentiation at the single-cell level using atomic force microscopic analysis

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Title
Monitoring of adipogenic differentiation at the single-cell level using atomic force microscopic analysis
Author(s)
Y N Kwon; Won Kon-Kim; S H Lee; K Kim; Eun Young Kim; Tai Hwan Ha; H Han; Kwang-Hee Bae
Bibliographic Citation
Spectroscopy, vol. 26, no. 6, pp. 329-335
Publication Year
2011
Abstract
Adipogenesis plays an important role in energy homeostasis by storing excess energy as lipid droplets. However, these reservoirs are implicated in a host of major human health problems, such as obesity. Elucidation of the mechanisms underlying adipogenesis is thus crucial to overcome these problems. The preadipocyte cell lines represent an optimal model to examine adipogenesis. Cells differentiate into adipocytes with various speeds of conversion and fat accumulation. Here, we have presented a novel method for detecting adipogenic differentiation at the single-cell level using atomic force microscopic analysis. Data obtained with this method revealed a good correlation between membrane stiffness and the degree of adipogenic differentiation. Although we could not determine the underlying cause for membrane stiffness reduction during adipogenic differentiation, the technique clearly offers advantages over the existing detection systems, such as lipid drop staining and extraction. Furthermore, the degree of adipogenic differentiation at the single-cell level can be detected with this method.
Keyword
AdipocyteadipogenesisAFMdifferentiationstiffness
ISSN
0887-6703
Publisher
Advanstar Communications Inc
DOI
http://dx.doi.org/10.3233/SPE-2012-0566
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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