CHO cells in biotechnology for production of recombinant proteins: current state and further potential = 치료용 단백질 생산을 위한 CHO세포의 연구 성과와 미래 연구 방향

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Title
CHO cells in biotechnology for production of recombinant proteins: current state and further potential = 치료용 단백질 생산을 위한 CHO세포의 연구 성과와 미래 연구 방향
Author(s)
J Y Kim; Yeon Gu Kim; G M Lee
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 93, no. 3, pp. 917-930
Publication Year
2012
Abstract
Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins. Recent advances in cell culture technology for rCHO cells have achieved significant improvement in protein production leading to titer of more than 10 g/L to meet the huge demand from market needs. This achievement is associated with progression in the establishment of high and stable producer and the optimization of culture process including media development. In this review article, we focus on current strategies and achievements in cell line development, mainly in vector engineering and cell engineering, for high and stable protein production in rCHO cells. The approaches that manipulate various DNA elements for gene targeting by site-specific integration and cis-acting elements to augment and stabilize gene expression are reviewed here. The genetic modulation strategy by "direct" cell engineering with growth-promoting and/or productivity-enhancing factors and omics-based approaches involved in transcriptomics, proteomics, and metabolomics to pursue cell engineering are also presented.
Keyword
Cell engineeringCell line developmentCHO cellsOmics-based approachesVector engineering
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00253-011-3758-5
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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