Virgibacillus campisalis sp. nov., from a marine solar saltern

Abstract

A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20 T, was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20 T grew optimally at 37 °C, at pH 7.5-8.0 and in the presence of 4- 5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20 T belongs to the genus Virgibacillus. Strain IDS-20 T exhibited 93.4-96.6% 16S rRNA gene sequence similarity to the type strains of species of the genus Virgibacillus. Strain IDS-20 T had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on meso-diaminopimelic acid. The major fatty acids were anteiso-C 15: 0 and anteiso-C 17: 0 and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20 T demonstrated that this strain can be distinguished from recognized species of the genus Virgibacillus. On the basis of the data presented, strain IDS-20 T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus campisalis sp. nov. is proposed. The type strain is IDS-20 T (=KCTC 13727 T =CCUG 59308 T).