Patulibacter ginsengiterrae sp. nov., isolated from soil of a ginseng field, and an emended description of the genus Patulibacter

Abstract

A novel actinobacterial strain, designated P4-5 T, was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P4-5 T were Gram-stain-positive, oxidase- and catalase-positive, motile, short rods and the strain produced creamy white colonies on trypticase soy agar. The isolate contained demethylmenaquinone 7 (DMK-7) as the predominant isoprenoid quinone, C 18: 1ω9c and anteiso-C 15: 0 as major fatty acids, diphosphatidylglycerol, phosphatidylglycerol and several unknown lipids in the polar lipid profile, galactose, glucose, mannose, arabinose, xylose (trace) and rhamnose as cell-wall sugars, and meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain P4-5 T was 74.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain P4-5 T was related most closely to Patulibacter minatonensis KV-614 T and Patulibacter americanus CP177-2 T (98.4 and 98.2% similarity, respectively) and that it formed a separate lineage in the genus Patulibacter. Combined phenotypic and DNA-DNA hybridization data supported the conclusion that strain P4-5 T represents a novel species of the genus Patulibacter, for which the name Patulibacter ginsengiterrae sp. nov. is proposed. The type strain is P4-5 T (=KCTC 19427 T =CECT 7603 T). An emended description of the genus Patulibacter is also provided.