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- Title
- Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation
- Author(s)
- J H Park; J H Lee; Chang-Jin Kim; Jae-Chan Lee; M H Cho; J Lee
- Bibliographic Citation
- Biotechnology Letters, vol. 34, no. 4, pp. 655-661
- Publication Year
- 2012
- Abstract
- Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcusaureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0. 1 μg proteinase K ml -1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.
- Keyword
- ActinomycetesBiofilmProteaseStaphylococcus aureus
- ISSN
- 0141-5492
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/s10529-011-0825-z
- Type
- Article
- Appears in Collections:
- 1. Journal Articles > Journal Articles
- Files in This Item:
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