Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

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Title
Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation
Author(s)
J H Park; J H Lee; Chang-Jin Kim; Jae-Chan Lee; M H Cho; J Lee
Bibliographic Citation
Biotechnology Letters, vol. 34, no. 4, pp. 655-661
Publication Year
2012
Abstract
Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcusaureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0. 1 μg proteinase K ml -1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.
Keyword
ActinomycetesBiofilmProteaseStaphylococcus aureus
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-011-0825-z
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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