DC Field | Value | Language |
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dc.contributor.author | Dong-Shan An | - |
dc.contributor.author | C H Cui | - |
dc.contributor.author | Bong Hyun Sung | - |
dc.contributor.author | H C Yang | - |
dc.contributor.author | S C Kim | - |
dc.contributor.author | S T Lee | - |
dc.contributor.author | W T Im | - |
dc.contributor.author | Song-Gun Kim | - |
dc.date.accessioned | 2017-04-19T09:29:29Z | - |
dc.date.available | 2017-04-19T09:29:29Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | 10.1007/s00253-011-3614-7 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10656 | - |
dc.description.abstract | The gene encoding an α-l-arabinofuranosidase that could biotransform ginsenosideRc {3-O-[β-d-glucopyranosyl-(1-2)-β-d-glucopyranosyl]-20-O-[α-l-arabinofuranosyl-(1-6)-β-d-glucopyranosyl]-20(S)-protopanaxadiol} to ginsenosideRd {3-O-[β-d-glucopyranosyl-(1-2)-β-d-glucopyranosyl]-20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol} was cloned from a soil bacterium, Rhodanobacter ginsenosidimutans strain Gsoil 3054 T, and the recombinant enzyme was characterized. The enzyme (AbfA) hydrolyzed the arabinofuranosyl moiety from ginsenoside Rc and was classified as a family 51 glycoside hydrolase based on amino acid sequence analysis. Recombinant AbfA expressed in Escherichia coli hydrolyzed non-reducing arabinofuranoside moieties with apparent K m values of 0.53 ± 0.07 and 0.30 ± 0.07 mM and V max values of 27.1 ± 1.7 and 49.6 ± 4.1 μmol min -1 mg -1 of protein for p-nitrophenyl-α-l-arabinofuranoside and ginsenoside Rc, respectively. The enzyme exhibited preferential substrate specificity of the exo-type mode of action towards polyarabinosides or oligoarabinosides. AbfA demonstrated substrate-specific activity for the bioconversion of ginsenosides, as it hydrolyzed only arabinofuranoside moieties from ginsenoside Rc and its derivatives, and not other sugar groups. These results are the first report of a glycoside hydrolase family 51 α-l-arabinofuranosidase that can transform ginsenoside Rc to Rd. | - |
dc.publisher | Springer | - |
dc.title | Characterization of a novel ginsenoside-hydrolyzing α-L-arabinofuranosidase, AbfA, from Rhodanobacter ginsenosidimutans Gsoil 3054T | - |
dc.title.alternative | Characterization of a novel ginsenoside-hydrolyzing α-L-arabinofuranosidase, AbfA, from Rhodanobacter ginsenosidimutans Gsoil 3054T | - |
dc.type | Article | - |
dc.citation.title | Applied Microbiology and Biotechnology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 682 | - |
dc.citation.startPage | 673 | - |
dc.citation.volume | 94 | - |
dc.contributor.affiliatedAuthor | Dong-Shan An | - |
dc.contributor.affiliatedAuthor | Bong Hyun Sung | - |
dc.contributor.affiliatedAuthor | Song-Gun Kim | - |
dc.contributor.alternativeName | 안동선 | - |
dc.contributor.alternativeName | 최창호 | - |
dc.contributor.alternativeName | 성봉현 | - |
dc.contributor.alternativeName | 양희찬 | - |
dc.contributor.alternativeName | 김선창 | - |
dc.contributor.alternativeName | 이성택 | - |
dc.contributor.alternativeName | 임완택 | - |
dc.contributor.alternativeName | 김성건 | - |
dc.identifier.bibliographicCitation | Applied Microbiology and Biotechnology, vol. 94, no. 3, pp. 673-682 | - |
dc.identifier.doi | 10.1007/s00253-011-3614-7 | - |
dc.subject.keyword | Biotransformation | - |
dc.subject.keyword | Compound Mc | - |
dc.subject.keyword | Compound Mc1 | - |
dc.subject.keyword | Ginseng saponin | - |
dc.subject.local | biotransformation | - |
dc.subject.local | Biotransformation | - |
dc.subject.local | Compound Mc | - |
dc.subject.local | Compound Mc1 | - |
dc.subject.local | Ginseng saponin | - |
dc.description.journalClass | Y | - |
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