Characterization of a novel ginsenoside-hydrolyzing α-L-arabinofuranosidase, AbfA, from Rhodanobacter ginsenosidimutans Gsoil 3054T

Cited 34 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorDong-Shan An-
dc.contributor.authorC H Cui-
dc.contributor.authorBong Hyun Sung-
dc.contributor.authorH C Yang-
dc.contributor.authorS C Kim-
dc.contributor.authorS T Lee-
dc.contributor.authorW T Im-
dc.contributor.authorSong-Gun Kim-
dc.date.accessioned2017-04-19T09:29:29Z-
dc.date.available2017-04-19T09:29:29Z-
dc.date.issued2012-
dc.identifier.issn0175-7598-
dc.identifier.uri10.1007/s00253-011-3614-7ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10656-
dc.description.abstractThe gene encoding an α-l-arabinofuranosidase that could biotransform ginsenosideRc {3-O-[β-d-glucopyranosyl-(1-2)-β-d-glucopyranosyl]-20-O-[α-l-arabinofuranosyl-(1-6)-β-d-glucopyranosyl]-20(S)-protopanaxadiol} to ginsenosideRd {3-O-[β-d-glucopyranosyl-(1-2)-β-d-glucopyranosyl]-20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol} was cloned from a soil bacterium, Rhodanobacter ginsenosidimutans strain Gsoil 3054 T, and the recombinant enzyme was characterized. The enzyme (AbfA) hydrolyzed the arabinofuranosyl moiety from ginsenoside Rc and was classified as a family 51 glycoside hydrolase based on amino acid sequence analysis. Recombinant AbfA expressed in Escherichia coli hydrolyzed non-reducing arabinofuranoside moieties with apparent K m values of 0.53 ± 0.07 and 0.30 ± 0.07 mM and V max values of 27.1 ± 1.7 and 49.6 ± 4.1 μmol min -1 mg -1 of protein for p-nitrophenyl-α-l-arabinofuranoside and ginsenoside Rc, respectively. The enzyme exhibited preferential substrate specificity of the exo-type mode of action towards polyarabinosides or oligoarabinosides. AbfA demonstrated substrate-specific activity for the bioconversion of ginsenosides, as it hydrolyzed only arabinofuranoside moieties from ginsenoside Rc and its derivatives, and not other sugar groups. These results are the first report of a glycoside hydrolase family 51 α-l-arabinofuranosidase that can transform ginsenoside Rc to Rd.-
dc.publisherSpringer-
dc.titleCharacterization of a novel ginsenoside-hydrolyzing α-L-arabinofuranosidase, AbfA, from Rhodanobacter ginsenosidimutans Gsoil 3054T-
dc.title.alternativeCharacterization of a novel ginsenoside-hydrolyzing α-L-arabinofuranosidase, AbfA, from Rhodanobacter ginsenosidimutans Gsoil 3054T-
dc.typeArticle-
dc.citation.titleApplied Microbiology and Biotechnology-
dc.citation.number3-
dc.citation.endPage682-
dc.citation.startPage673-
dc.citation.volume94-
dc.contributor.affiliatedAuthorDong-Shan An-
dc.contributor.affiliatedAuthorBong Hyun Sung-
dc.contributor.affiliatedAuthorSong-Gun Kim-
dc.contributor.alternativeName안동선-
dc.contributor.alternativeName최창호-
dc.contributor.alternativeName성봉현-
dc.contributor.alternativeName양희찬-
dc.contributor.alternativeName김선창-
dc.contributor.alternativeName이성택-
dc.contributor.alternativeName임완택-
dc.contributor.alternativeName김성건-
dc.identifier.bibliographicCitationApplied Microbiology and Biotechnology, vol. 94, no. 3, pp. 673-682-
dc.identifier.doi10.1007/s00253-011-3614-7-
dc.subject.keywordBiotransformation-
dc.subject.keywordCompound Mc-
dc.subject.keywordCompound Mc1-
dc.subject.keywordGinseng saponin-
dc.subject.localbiotransformation-
dc.subject.localBiotransformation-
dc.subject.localCompound Mc-
dc.subject.localCompound Mc1-
dc.subject.localGinseng saponin-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.