DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung Hwa Lim | - |
dc.contributor.author | Hyo-Jung Shin | - |
dc.contributor.author | Kyeong Su Park | - |
dc.contributor.author | C H Lee | - |
dc.contributor.author | Cho-Rok Jung | - |
dc.contributor.author | Dong Soo Im | - |
dc.date.accessioned | 2017-04-19T09:29:29Z | - |
dc.date.available | 2017-04-19T09:29:29Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1525-0016 | - |
dc.identifier.uri | 10.1038/mt.2011.302 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10659 | - |
dc.description.abstract | E2-EPF ubiquitin carrier protein (UCP) stabilizes hypoxia-inducible factor-1α (HIF-1α) inducing ischemic vascular responses. Here, we investigated the effect of UCP gene transfer on therapeutic angiogenesis. Adenovirus-encoded UCP (Ad-F-UCP) increased the expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) in cells and mice. Conditioned media from UCP-overexpressing cells promoted proliferation, tubule formation, and invasion of human umbilical-vascular- endothelial cells (HUVECs), and vascularization in chorioallantoic membrane (CAM) assay. Ad-F-UCP increased the vessel density in the Martigel plug assay, and generated copious vessel-like structures in the explanted muscle. The UCP effect on angiogenesis was dependent on VEGF and FGF-2. In mouse hindlimb ischemia model (N = 30/group), autoamputation (limb loss) occurred in 87% and 68% of the mice with saline and Ad encoding Β-galactosidase (Ad-LacZ), respectively, whereas only 23% of the mice injected with Ad-F-UCP showed autoamputation after 21 days of treatment. Ad-F-UCP increased protein levels of HIF-1α, platelet-endothelial cell adhesion molecule-1 (PECAM-1), smooth muscle cell actin (SMA) in the ischemic muscle, and augmented blood vessels doubly positive for PECAM-1 and SMA. Consequently, UCP gene transfer prevented muscle degeneration and autoamputation of ischemic limb. The results suggest that E2-EPF UCP may be a target for therapeutic angiogenesis. | - |
dc.publisher | Elsevier-Cell Press | - |
dc.title | Adenovirus-mediated E2-EPF UCP gene transfer prevents autoamputation in a mouse model of hindlimb ischemia | - |
dc.title.alternative | Adenovirus-mediated E2-EPF UCP gene transfer prevents autoamputation in a mouse model of hindlimb ischemia | - |
dc.type | Article | - |
dc.citation.title | Molecular Therapy | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 787 | - |
dc.citation.startPage | 778 | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Jung Hwa Lim | - |
dc.contributor.affiliatedAuthor | Hyo-Jung Shin | - |
dc.contributor.affiliatedAuthor | Kyeong Su Park | - |
dc.contributor.affiliatedAuthor | Cho-Rok Jung | - |
dc.contributor.affiliatedAuthor | Dong Soo Im | - |
dc.contributor.alternativeName | 임정화 | - |
dc.contributor.alternativeName | 신효정 | - |
dc.contributor.alternativeName | 박경수 | - |
dc.contributor.alternativeName | 이찬희 | - |
dc.contributor.alternativeName | 정초록 | - |
dc.contributor.alternativeName | 임동수 | - |
dc.identifier.bibliographicCitation | Molecular Therapy, vol. 20, no. 4, pp. 778-787 | - |
dc.identifier.doi | 10.1038/mt.2011.302 | - |
dc.description.journalClass | Y | - |
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