Characterization of the core oligosaccharide and the O-antigen biological repeating unit from Halomonas stevensii lipopolysaccharide: The first case of O-antigen linked to the inner core

Cited 10 time in scopus
Metadata Downloads
Title
Characterization of the core oligosaccharide and the O-antigen biological repeating unit from Halomonas stevensii lipopolysaccharide: The first case of O-antigen linked to the inner core
Author(s)
G Pieretti; S Carillo; B Lindner; Kwang Kyu Kim; Keun Chul LeeJung-Sook Lee; R Lanzetta; M Parrilli; M M Corsaro
Bibliographic Citation
Chemistry-A European Journal, vol. 18, no. 12, pp. 3729-3735
Publication Year
2012
Abstract
A novel core structure among bacterial lipopolysaccharides (LPS) that belong to the genus Halomonas has been characterized. H. stevensii is a moderately halophilic microorganism, as are the majority of the Halomonadaceae. It brought to light the pathogenic potential of this genus. On account of their role in immune system elicitation, elucidation of LPS structure is the mandatory starting point for a deeper understanding of the interaction mechanisms between host and pathogen. In this paper we report the structure of the complete saccharidic portion of the LPS from H. stevensii. In contrast to the finding that the O-antigen is usually covalently linked to the outer core oligosaccharide, we could demonstrate that the O-polysaccharide of H. stevensii is linked to the inner core of an LPS. By means of high-performance anion-exchange chromatography with pulsed amperometric detection we were able to isolate the core decasaccharide as well as a tridecasaccharide constituted by the core region plus one O-repeating unit after alkaline degradation of the LPS. The structure was elucidated by one- and two-dimensional NMR spectroscopy, ESI Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry, and chemical analysis.
Keyword
bacteriacarbohydratesHalomonas stevensiiNMR spectroscopystructure elucidation
ISSN
0947-6539
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/chem.201102550
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.