Biochemical characterization of thermophilic dextranase from a thermophilic bacterium, Thermoanaerobacter pseudethanolicus = 고온성 미생물로부터 내열성 글루칸 분해효소의 생화학적 특성

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Title
Biochemical characterization of thermophilic dextranase from a thermophilic bacterium, Thermoanaerobacter pseudethanolicus = 고온성 미생물로부터 내열성 글루칸 분해효소의 생화학적 특성
Author(s)
Tae Soon Park; Hyung Jae Jung; Jin A Ko; Young Bae RyuSu-Jin Park; D Kim; Young Min Kim; Woo Song Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 22, no. 5, pp. 637-641
Publication Year
2012
Abstract
TPDex, a putative dextranase from Thermoanaerobacter pseudethanolicus, was purified as a single 70 kDa band of 7.37 U/mg. Its optimum pH was 5.2 and the enzyme was stable between pH 3.1 and 8.5 at 70°C. A half-life comparison showed that TPDex was stable for 7.4 h at 70°C, whereas Chaetominum dextranase (CEDex), currently used as a dextranase for sugar milling, was stable at 55°C. TPDex showed broad dextranase activity regardless of dextran types, including dextran T2000, 742CB dextran, and alternan. TPDex showed the highest thermostability among the characterized dextranases, and may be a suitable enzyme for use in sugar manufacture without decreased temperature.
Keyword
Sugar processingThermoanaereobacterThermostabilityThermostable dextranase
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1112.12024
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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