Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system = E. coli strain과 유도제의 조합에 의한 글루칸 합성효소의 대량발현

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dc.contributor.authorJin A Ko-
dc.contributor.authorHyung Jae Jung-
dc.contributor.authorYoung Bae Ryu-
dc.contributor.authorSu-Jin Park-
dc.contributor.authorY J Wee-
dc.contributor.authorD Kim-
dc.contributor.authorYoung Min Kim-
dc.contributor.authorWoo Song Lee-
dc.date.accessioned2017-04-19T09:29:36Z-
dc.date.available2017-04-19T09:29:36Z-
dc.date.issued2012-
dc.identifier.issn1017-7825-
dc.identifier.uri10.4014/jmb.1111.11032ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10677-
dc.description.abstractA recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD 600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17°C. These results may effectively apply to the heterologous expression of other large DexT genes.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleLarge increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system = E. coli strain과 유도제의 조합에 의한 글루칸 합성효소의 대량발현-
dc.title.alternativeLarge increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number4-
dc.citation.endPage515-
dc.citation.startPage510-
dc.citation.volume22-
dc.contributor.affiliatedAuthorJin A Ko-
dc.contributor.affiliatedAuthorHyung Jae Jung-
dc.contributor.affiliatedAuthorYoung Bae Ryu-
dc.contributor.affiliatedAuthorSu-Jin Park-
dc.contributor.affiliatedAuthorYoung Min Kim-
dc.contributor.affiliatedAuthorWoo Song Lee-
dc.contributor.alternativeName고진아-
dc.contributor.alternativeName정형재-
dc.contributor.alternativeName류영배-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName위영중-
dc.contributor.alternativeName김도만-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName이우송-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 22, no. 4, pp. 510-515-
dc.identifier.doi10.4014/jmb.1111.11032-
dc.subject.keywordDextransucrase-
dc.subject.keywordLactose-
dc.subject.keywordLeuconostoc citreum KM20-
dc.subject.keywordOptimization-
dc.subject.keywordResponse surface methodology-
dc.subject.localDextransucrase-
dc.subject.localLactose-
dc.subject.locallactose-
dc.subject.localLeuconostoc citreum KM20-
dc.subject.localoptimization-
dc.subject.localOptimization-
dc.subject.localResponse-surface methodology-
dc.subject.localresponse surface methodology-
dc.subject.localResponse surface methodology-
dc.subject.localResponse Surface Methodology (RSM)-
dc.description.journalClassY-
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Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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