Effective screening of Scenedesmus sp. from environmental microalgae communities using optimal sonication conditions predicted by statistical parameters of fluorescence-activated cell sorting

Cited 7 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHyun Joon La-
dc.contributor.authorJae Yon Lee-
dc.contributor.authorSong-Gun Kim-
dc.contributor.authorGang Guk Choi-
dc.contributor.authorChi-Yong Ahn-
dc.contributor.authorHee-Mock Oh-
dc.date.accessioned2017-04-19T09:29:55Z-
dc.date.available2017-04-19T09:29:55Z-
dc.date.issued2012-
dc.identifier.issn0960-8524-
dc.identifier.uri10.1016/j.biortech.2012.02.140ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10704-
dc.description.abstractThe effects of the sonication parameters, including the power and time, were investigated for the effective isolation of Scenedesmus sp. from environmental microalgae communities when using fluorescence-activated cell sorting (FACS). The selectivity, defined as the percentage of Scenedesmus sp. successfully isolated and grown in microplates, appeared as peaks in contour plots spanned by the sonication power and time. For fast screening of the optimal sonication conditions, correlations between the selectivity and the statistical parameters from the FACS analysis were investigated. A graphical comparison analysis of the contour plots showed a pattern similarity of over 82% between the coefficients of variation for the side scatter (SSC-CV) and the selectivity. This predictability of the optimal sonication conditions enabled a Scenedesmus sp. selectivity of ca. 2. times using only one-third of the sonication condition sets arbitrarily chosen around the peaks of the SSC-CV, thereby saving resources and time for subsequent processes.-
dc.publisherElsevier-
dc.titleEffective screening of Scenedesmus sp. from environmental microalgae communities using optimal sonication conditions predicted by statistical parameters of fluorescence-activated cell sorting-
dc.title.alternativeEffective screening of Scenedesmus sp. from environmental microalgae communities using optimal sonication conditions predicted by statistical parameters of fluorescence-activated cell sorting-
dc.typeArticle-
dc.citation.titleBioresource Technology-
dc.citation.number0-
dc.citation.endPage483-
dc.citation.startPage478-
dc.citation.volume114-
dc.contributor.affiliatedAuthorHyun Joon La-
dc.contributor.affiliatedAuthorJae Yon Lee-
dc.contributor.affiliatedAuthorSong-Gun Kim-
dc.contributor.affiliatedAuthorGang Guk Choi-
dc.contributor.affiliatedAuthorChi-Yong Ahn-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.alternativeName나현준-
dc.contributor.alternativeName이재연-
dc.contributor.alternativeName김성건-
dc.contributor.alternativeName최강국-
dc.contributor.alternativeName안치용-
dc.contributor.alternativeName오희목-
dc.identifier.bibliographicCitationBioresource Technology, vol. 114, pp. 478-483-
dc.identifier.doi10.1016/j.biortech.2012.02.140-
dc.subject.keywordCoefficient of variation-
dc.subject.keywordEnvironmental microalgae communities-
dc.subject.keywordFACS-
dc.subject.keywordScenedesmus sp.-
dc.subject.keywordSonication-
dc.subject.localCoefficient of variation-
dc.subject.localEnvironmental microalgae communities-
dc.subject.localFACS-
dc.subject.localScenedesmus sp-
dc.subject.localScenedesmus sp.-
dc.subject.localSonication-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.