Protein tyrosine phosphatase profiling analysis of HIB-1B cells during brown adipogenesis

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Title
Protein tyrosine phosphatase profiling analysis of HIB-1B cells during brown adipogenesis
Author(s)
Hye-Ryung Choi; Won Kon Kim; Eun Young Kim; Hyeyun Jung; Jeong Hoon KimBaek Soo Han; K H You; Sang Chul LeeKwang-Hee Bae
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 22, no. 7, pp. 1029-1033
Publication Year
2012
Abstract
A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the "PTPome." In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.
Keyword
Brown adipogenesisProtein tyrosine phosphatasePTP-REPTPome
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1112.12059
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
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