Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells

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dc.contributor.authorSun Young Kim-
dc.contributor.authorMin-Jeong Kim-
dc.contributor.authorHyeyun Jung-
dc.contributor.authorWon Kon Kim-
dc.contributor.authorS O Kwon-
dc.contributor.authorMyung Jin Son-
dc.contributor.authorI S Jang-
dc.contributor.authorJ S Choi-
dc.contributor.authorSung Goo Park-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorY M Han-
dc.contributor.authorSang Chul Lee-
dc.contributor.authorYee Sook Cho-
dc.contributor.authorKwang-Hee Bae-
dc.date.accessioned2017-04-19T09:30:03Z-
dc.date.available2017-04-19T09:30:03Z-
dc.date.issued2012-
dc.identifier.issn1525-8165-
dc.identifier.uri10.1089/scd.2011.0243ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10714-
dc.description.abstractInduced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we performed comparative proteomic analyses of human somatic cells [human newborn foreskin fibroblasts (hFFs)], human iPSCs (hiPSCs) derived from hFFs, and H9 human ESCs (hESCs). We reprogrammed hFFs to a pluripotent state using 4 core transcription factors: Oct4 (O), Sox2 (S), Klf4 (K), and c-Myc (M). The proteome of hiPSCs induced by 4 core transcription factors was relatively similar to that of hESCs. However, several proteins, including dUTPase, GAPDH, and FUSE binding protein 3, were differentially expressed between hESCs and hiPSCs, implying that hiPSCs are not identical to hESCs at the proteomic level. The proteomes of iPSCs induced by introducing 3, 5, or 6 transcription factors were also analyzed. Our proteomic profiles provide valuable insight into the factors that contribute to the similarities and differences between hESCs and hiPSCs and the mechanisms of reprogramming.-
dc.publisherMary Ann Liebert, Inc-
dc.titleComparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells-
dc.title.alternativeComparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells-
dc.typeArticle-
dc.citation.titleStem Cells and Development-
dc.citation.number8-
dc.citation.endPage1286-
dc.citation.startPage1272-
dc.citation.volume21-
dc.contributor.affiliatedAuthorSun Young Kim-
dc.contributor.affiliatedAuthorMin-Jeong Kim-
dc.contributor.affiliatedAuthorHyeyun Jung-
dc.contributor.affiliatedAuthorWon Kon Kim-
dc.contributor.affiliatedAuthorMyung Jin Son-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorSang Chul Lee-
dc.contributor.affiliatedAuthorYee Sook Cho-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName김민정-
dc.contributor.alternativeName정혜윤-
dc.contributor.alternativeName김원곤-
dc.contributor.alternativeName권상오-
dc.contributor.alternativeName손명진-
dc.contributor.alternativeName장익순-
dc.contributor.alternativeName최종순-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName이상철-
dc.contributor.alternativeName조이숙-
dc.contributor.alternativeName배광희-
dc.identifier.bibliographicCitationStem Cells and Development, vol. 21, no. 8, pp. 1272-1286-
dc.identifier.doi10.1089/scd.2011.0243-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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