Detection and molecular chracterization of porcine type 3 orthoreoviruses circulating in South Korea

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dc.contributor.authorHyung Jun Kwon-
dc.contributor.authorHa-Hyun Kim-
dc.contributor.authorH J Kim-
dc.contributor.authorJ G Park-
dc.contributor.authorK Y Son-
dc.contributor.authorJuyeon Jung-
dc.contributor.authorWoo Song Lee-
dc.contributor.authorK O Cho-
dc.contributor.authorSu-Jin Park-
dc.contributor.authorM I Kang-
dc.date.accessioned2017-04-19T09:30:03Z-
dc.date.available2017-04-19T09:30:03Z-
dc.date.issued2012-
dc.identifier.issn0378-1135-
dc.identifier.uri10.1016/j.vetmic.2011.12.032ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10718-
dc.description.abstractOrthoreoviruses infect virtually all mammalian species, causing systemic infections including mild gastrointestinal and respiratory illnesses. However, little is known about the prevalence or genetic diversity of porcine orthoreoviruses in South Korea. We examined 237 diarrheic fecal samples collected from 78 pig farms around the country. RT-PCR utilizing primers specific for the L1 gene of mammalian orthoreoviruses showed that 45 (19.0%) samples were positive. The 10 strains isolated from orthoreovirus-positive samples formed typical perinuclear cytoplasmic inclusion bodies and had an atypical hemagglutination pattern; these are characteristics of type 3 orthoreovirus. Phylogenetic analysis of the S1 gene in these 10 Korean and other strains showed that type 3 orthoreoviruses could be divided into four lineages; the 10 Korean strains were included in porcine lineage IV, along with T3/porcine/Sichuan/2006. Sequence analysis showed that strains in lineage IV had nucleotide identities of 97.0-98.1% and deduced amino acid identities of 96.4-98.2%. Sequence analysis of the σ1 protein, a viral attachment protein, revealed that the amino acid sequences associated with neurotropism (amino acids 198-204, 249I, 350D, and 419E) were highly conserved among the Korean strains, confirming that neural tropism was present. In conclusion, our findings suggest that porcine orthoreovirus infections are endemic in pig farms in South Korea and that the 10 novel Korean porcine orthoreoviruses belong to porcine lineage IV of type 3 orthoreovirus. In addition, sequence analysis of S1 genes encoding the σ1 protein showed that the 9 of 10 Korean porcine orthoreoviruses exhibited neural tropism.-
dc.publisherElsevier-
dc.titleDetection and molecular chracterization of porcine type 3 orthoreoviruses circulating in South Korea-
dc.title.alternativeDetection and molecular chracterization of porcine type 3 orthoreoviruses circulating in South Korea-
dc.typeArticle-
dc.citation.titleVeterinary Microbiology-
dc.citation.number3-
dc.citation.endPage463-
dc.citation.startPage456-
dc.citation.volume157-
dc.contributor.affiliatedAuthorHyung Jun Kwon-
dc.contributor.affiliatedAuthorHa-Hyun Kim-
dc.contributor.affiliatedAuthorJuyeon Jung-
dc.contributor.affiliatedAuthorWoo Song Lee-
dc.contributor.affiliatedAuthorSu-Jin Park-
dc.contributor.alternativeName권형준-
dc.contributor.alternativeName김하현-
dc.contributor.alternativeName김현정-
dc.contributor.alternativeName박준규-
dc.contributor.alternativeName손규열-
dc.contributor.alternativeName정주연-
dc.contributor.alternativeName이우송-
dc.contributor.alternativeName조경오-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName강문일-
dc.identifier.bibliographicCitationVeterinary Microbiology, vol. 157, no. 3, pp. 456-463-
dc.identifier.doi10.1016/j.vetmic.2011.12.032-
dc.subject.keywordGenetic diversity-
dc.subject.keywordOrthoreovirus-
dc.subject.keywordPorcine-
dc.subject.keywordPrevalence-
dc.subject.localgenetic diversity-
dc.subject.localGenetic diversity-
dc.subject.localOrthoreovirus-
dc.subject.localPorcine-
dc.subject.localporcine-
dc.subject.localprevalence-
dc.subject.localPrevalence-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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