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- Title
- A screening method for β-glucan hydrolase employing Trypan Blue-coupled β-glucan agar plate and β-glucan zymography
- Author(s)
- C S Park; Hee Jong Yang; D H Kim; D O Kang; Min-Soo Kim; N S Choi
- Bibliographic Citation
- Biotechnology Letters, vol. 34, no. 6, pp. 1073-1077
- Publication Year
- 2012
- Abstract
- A new screening method for β-(1,3-1,6) glucan hydrolase was developed using a pure β-glucan from Aureobaisidum pullulans by zymography and an LB-agar plate. Paenibacillus sp. was screened as a producer a β-glucan hydrolase on the Trypan Blue-coupled β-glucan LB-agar plate and the activity of the enzyme was analyzed by SDS-β-glucan zymography. The β-glucan was not hydrolyzed by Bacillus spp. strains, which exhibit cellulolytic activity on CMC zymography. The gene, obtaining by shotgun cloning and encoding the β-glucan hydrolase of Paenibacillus sp. was sequenced.
- Keyword
- β-Glucan hydrolasePaenibacillus spShotgun cloningZymography
- ISSN
- 0141-5492
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1007/s10529-012-0873-z
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
- Files in This Item:
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