A screening method for β-glucan hydrolase employing Trypan Blue-coupled β-glucan agar plate and β-glucan zymography

Cited 4 time in scopus
Metadata Downloads
Title
A screening method for β-glucan hydrolase employing Trypan Blue-coupled β-glucan agar plate and β-glucan zymography
Author(s)
C S Park; Hee Jong Yang; D H Kim; D O Kang; Min-Soo Kim; N S Choi
Bibliographic Citation
Biotechnology Letters, vol. 34, no. 6, pp. 1073-1077
Publication Year
2012
Abstract
A new screening method for β-(1,3-1,6) glucan hydrolase was developed using a pure β-glucan from Aureobaisidum pullulans by zymography and an LB-agar plate. Paenibacillus sp. was screened as a producer a β-glucan hydrolase on the Trypan Blue-coupled β-glucan LB-agar plate and the activity of the enzyme was analyzed by SDS-β-glucan zymography. The β-glucan was not hydrolyzed by Bacillus spp. strains, which exhibit cellulolytic activity on CMC zymography. The gene, obtaining by shotgun cloning and encoding the β-glucan hydrolase of Paenibacillus sp. was sequenced.
Keyword
β-Glucan hydrolasePaenibacillus spShotgun cloningZymography
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-012-0873-z
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.