DC Field | Value | Language |
---|---|---|
dc.contributor.author | Soo-Young Park | - |
dc.contributor.author | Seung Hwan Park | - |
dc.contributor.author | Soo Keun Choi | - |
dc.date.accessioned | 2017-04-19T09:30:04Z | - |
dc.date.available | 2017-04-19T09:30:04Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | 10.1016/j.ab.2012.04.002 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10721 | - |
dc.description.abstract | Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coli. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli. | - |
dc.publisher | Elsevier | - |
dc.title | Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli | - |
dc.title.alternative | Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Analytical Biochemistry | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 65 | - |
dc.citation.startPage | 63 | - |
dc.citation.volume | 426 | - |
dc.contributor.affiliatedAuthor | Soo-Young Park | - |
dc.contributor.affiliatedAuthor | Seung Hwan Park | - |
dc.contributor.affiliatedAuthor | Soo Keun Choi | - |
dc.contributor.alternativeName | 박수영 | - |
dc.contributor.alternativeName | 박승환 | - |
dc.contributor.alternativeName | 최수근 | - |
dc.identifier.bibliographicCitation | Analytical Biochemistry, vol. 426, no. 1, pp. 63-65 | - |
dc.identifier.doi | 10.1016/j.ab.2012.04.002 | - |
dc.subject.keyword | Active inclusion body | - |
dc.subject.keyword | Paenibacillus polymyxa | - |
dc.subject.keyword | PoxB | - |
dc.subject.keyword | Recombinant protein expression | - |
dc.subject.local | Active inclusion body | - |
dc.subject.local | Paenibacillus polymyxa | - |
dc.subject.local | paenibacillus polymyxa | - |
dc.subject.local | PoxB | - |
dc.subject.local | Recombinant protein expression | - |
dc.subject.local | recombinant protein expression | - |
dc.description.journalClass | Y | - |
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