Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris = 플라보노이드 유래 SARS coronavirus 3CL protease 저해 특성

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dc.contributor.authorT T H Nguyen-
dc.contributor.authorH J Woo-
dc.contributor.authorH K Kang-
dc.contributor.authorV D Nguyen-
dc.contributor.authorYoung Min Kim-
dc.contributor.authorD W Kim-
dc.contributor.authorS A Ahn-
dc.contributor.authorY Xia-
dc.contributor.authorD Kim-
dc.date.accessioned2017-04-19T09:30:16Z-
dc.date.available2017-04-19T09:30:16Z-
dc.date.issued2012-
dc.identifier.issn0141-5492-
dc.identifier.uri10.1007/s10529-011-0845-8ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10726-
dc.description.abstractThe 3C-like protease (3CL pro) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CL pro was expressed in Pichia pastoris GS115 as a 42 kDa protein that displayed a K m of 15 ± 2 μM with Dabcyl-KTSAVLQSGFRKME-Edans as substrate. Purified 3CL pro was used for inhibition and kinetic assays with seven flavonoid compounds. The IC 50 of six flavonoidcompounds were 47-381 μM. Quercetin, epigallocatechin gallate and gallocatechin gallate (GCG) displayed good inhibition toward 3CL pro with IC 50 values of 73, 73 and 47 μM, respectively. GCG showed a competitive inhibition pattern with K i value of 25 ± 1. 7 μM. In molecular docking experiments, GCG displayed a binding energy of -14 kcal mol -1 to the active site of 3CL pro and the galloyl moiety at 3-OH position was required for 3CL pro inhibition activity.-
dc.publisherSpringer-
dc.titleFlavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris = 플라보노이드 유래 SARS coronavirus 3CL protease 저해 특성-
dc.title.alternativeFlavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number5-
dc.citation.endPage838-
dc.citation.startPage831-
dc.citation.volume34-
dc.contributor.affiliatedAuthorYoung Min Kim-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName우혜진-
dc.contributor.alternativeName강희경-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName김도원-
dc.contributor.alternativeName안설아-
dc.contributor.alternativeNameXia-
dc.contributor.alternativeName김도만-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 34, no. 5, pp. 831-838-
dc.identifier.doi10.1007/s10529-011-0845-8-
dc.subject.keywordFlavonoid-
dc.subject.keywordMolecular docking-
dc.subject.keywordPichia pastoris-
dc.subject.keywordProtease-
dc.subject.keywordSevere acute respiratory syndrome (SARS)-
dc.subject.localFlavonoids-
dc.subject.localflavonoid-
dc.subject.localflavonoids-
dc.subject.localFlavonoid-
dc.subject.localmolecular docking-
dc.subject.localMolecular docking-
dc.subject.localpichia pastoris-
dc.subject.localPichia pastoris-
dc.subject.localprotease-
dc.subject.localProtease-
dc.subject.localProteases-
dc.subject.localSevere acute respiratory syndrome (SARS)-
dc.subject.localSevere acute respiratory syndrome-
dc.subject.localsevere acute respiratory syndrome-
dc.description.journalClassY-
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