DC Field | Value | Language |
---|---|---|
dc.contributor.author | T T H Nguyen | - |
dc.contributor.author | H J Woo | - |
dc.contributor.author | H K Kang | - |
dc.contributor.author | V D Nguyen | - |
dc.contributor.author | Young Min Kim | - |
dc.contributor.author | D W Kim | - |
dc.contributor.author | S A Ahn | - |
dc.contributor.author | Y Xia | - |
dc.contributor.author | D Kim | - |
dc.date.accessioned | 2017-04-19T09:30:16Z | - |
dc.date.available | 2017-04-19T09:30:16Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | 10.1007/s10529-011-0845-8 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10726 | - |
dc.description.abstract | The 3C-like protease (3CL pro) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CL pro was expressed in Pichia pastoris GS115 as a 42 kDa protein that displayed a K m of 15 ± 2 μM with Dabcyl-KTSAVLQSGFRKME-Edans as substrate. Purified 3CL pro was used for inhibition and kinetic assays with seven flavonoid compounds. The IC 50 of six flavonoidcompounds were 47-381 μM. Quercetin, epigallocatechin gallate and gallocatechin gallate (GCG) displayed good inhibition toward 3CL pro with IC 50 values of 73, 73 and 47 μM, respectively. GCG showed a competitive inhibition pattern with K i value of 25 ± 1. 7 μM. In molecular docking experiments, GCG displayed a binding energy of -14 kcal mol -1 to the active site of 3CL pro and the galloyl moiety at 3-OH position was required for 3CL pro inhibition activity. | - |
dc.publisher | Springer | - |
dc.title | Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris = 플라보노이드 유래 SARS coronavirus 3CL protease 저해 특성 | - |
dc.title.alternative | Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Letters | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 838 | - |
dc.citation.startPage | 831 | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Young Min Kim | - |
dc.contributor.alternativeName | Nguyen | - |
dc.contributor.alternativeName | 우혜진 | - |
dc.contributor.alternativeName | 강희경 | - |
dc.contributor.alternativeName | Nguyen | - |
dc.contributor.alternativeName | 김영민 | - |
dc.contributor.alternativeName | 김도원 | - |
dc.contributor.alternativeName | 안설아 | - |
dc.contributor.alternativeName | Xia | - |
dc.contributor.alternativeName | 김도만 | - |
dc.identifier.bibliographicCitation | Biotechnology Letters, vol. 34, no. 5, pp. 831-838 | - |
dc.identifier.doi | 10.1007/s10529-011-0845-8 | - |
dc.subject.keyword | Flavonoid | - |
dc.subject.keyword | Molecular docking | - |
dc.subject.keyword | Pichia pastoris | - |
dc.subject.keyword | Protease | - |
dc.subject.keyword | Severe acute respiratory syndrome (SARS) | - |
dc.subject.local | Flavonoids | - |
dc.subject.local | flavonoid | - |
dc.subject.local | flavonoids | - |
dc.subject.local | Flavonoid | - |
dc.subject.local | molecular docking | - |
dc.subject.local | Molecular docking | - |
dc.subject.local | pichia pastoris | - |
dc.subject.local | Pichia pastoris | - |
dc.subject.local | protease | - |
dc.subject.local | Protease | - |
dc.subject.local | Proteases | - |
dc.subject.local | Severe acute respiratory syndrome (SARS) | - |
dc.subject.local | Severe acute respiratory syndrome | - |
dc.subject.local | severe acute respiratory syndrome | - |
dc.description.journalClass | Y | - |
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