Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform

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Title
Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform
Author(s)
Hyo Jin Kang; Y J Kang; Young-Mi Lee; H H Shin; Sang Jeon Chung; S Kang
Bibliographic Citation
Biomaterials, vol. 33, no. 21, pp. 5423-5430
Publication Year
2012
Abstract
We genetically introduced the Fc-binding peptide (FcBP) into the loop of a self-assembled protein cage, ferritin, constituting four-fold symmetry at the surface to use it as a modular delivery nanoplatform. FcBP-presenting ferritin (FcBP-ferritin) formed very stable non-covalent complexes with both human and rabbit IgGs through the simple molecular recognition between the Fc region of the antibodies and the Fc-binding peptide clusters inserted onto the surface of FcBP-ferritin. This approach realized orientation-controlled display of antibodies on the surfaces of the protein cages simply by mixing without any complicated chemical conjugation. Using trastuzumab, a human anti-HER2 antibody used to treat patients with breast cancer, and a rabbit antibody to folate receptor, along with fluorescently labeled FcBP-ferritin, we demonstrated the specific binding of these complexes to breast cancer cells and folate receptor over-expressing cells, respectively, by fluorescent cell imaging. FcBP-ferritin may be potentially used as modular nanoplatforms for active targeted delivery vehicles or molecular imaging probes with a series of antibodies on demand.
Keyword
Antibody-bindingDelivery platformFc-binding peptideMolecular recognitionProtein cages
ISSN
0142-9612
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.biomaterials.2012.03.055
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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