Direct transcriptional regulation of Six6 is controlled by SoxB1 binding to a remote forebrain enhancer

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dc.contributor.authorB Lee-
dc.contributor.authorK Rizzoti-
dc.contributor.authorD S Kwon-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorS Oh-
dc.contributor.authorD J Epstein-
dc.contributor.authorY Son-
dc.contributor.authorJ Yoon-
dc.contributor.authorK Baek-
dc.contributor.authorY Jeong-
dc.date.accessioned2017-04-19T09:30:40Z-
dc.date.available2017-04-19T09:30:40Z-
dc.date.issued2012-
dc.identifier.issn0012-1606-
dc.identifier.uri10.1016/j.ydbio.2012.04.023ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10750-
dc.description.abstractSix6, a sine oculis homeobox protein, plays a crucial and conserved role in the development of the forebrain and eye. To understand how the expression of Six6 is regulated during embryogenesis, we screened ~250. kb of genomic DNA encompassing the Six6 locus for cis-regulatory elements capable of directing reporter gene expression to sites of Six6 transcription in transgenic mouse embryos. Here, we describe two novel enhancer elements, that are highly conserved in vertebrate species and whose activities recapitulate Six6 expression in the ventral forebrain and eye, respectively. Cross-species comparisons of the Six6 forebrain enhancer sequences revealed highly conserved binding sites matching the consensus for homeodomain and SoxB1 transcription factors. Deletion of either of the binding sites resulted in loss of the forebrain enhancer activity in the ventral forebrain. Moreover, our studies show that members of the SoxB1 family, including Sox2 and Sox3, are expressed in the overlapping region of the ventral forebrain with Six6 and can bind to the Six6 forebrain enhancer. Loss of function of SoxB1 genes in vivo further emphasizes their role in regulating Six6 forebrain enhancer activity. Thus, our data strongly suggest that SoxB1 transcription factors are direct activators of Six6 expression in the ventral forebrain.-
dc.publisherElsevier-
dc.titleDirect transcriptional regulation of Six6 is controlled by SoxB1 binding to a remote forebrain enhancer-
dc.title.alternativeDirect transcriptional regulation of Six6 is controlled by SoxB1 binding to a remote forebrain enhancer-
dc.typeArticle-
dc.citation.titleDevelopmental Biology-
dc.citation.number2-
dc.citation.endPage403-
dc.citation.startPage393-
dc.citation.volume366-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.alternativeName이범희-
dc.contributor.alternativeNameRizzoti-
dc.contributor.alternativeName-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName오상택-
dc.contributor.alternativeNameEpstein-
dc.contributor.alternativeName손영숙-
dc.contributor.alternativeName윤재승-
dc.contributor.alternativeName백광희-
dc.contributor.alternativeName정용수-
dc.identifier.bibliographicCitationDevelopmental Biology, vol. 366, no. 2, pp. 393-403-
dc.identifier.doi10.1016/j.ydbio.2012.04.023-
dc.subject.keywordForebrain-
dc.subject.keywordGene expression-
dc.subject.keywordSix6-
dc.subject.keywordSoxB1-
dc.subject.localForebrain-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localSix6-
dc.subject.localSoxB1-
dc.description.journalClassY-
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