Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii

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Title
Simple, rapid and cost-effective method for high quality nucleic acids extraction from different strains of Botryococcus braunii
Author(s)
Byung Hyuk Kim; R Ramanan; Dae Hyun Cho; G G Choi; Hyun Joon La; Chi-Yong AhnHee-Mock OhHee-Sik Kim
Bibliographic Citation
PLoS One, vol. 7, no. 5, pp. e37770-e37770
Publication Year
2012
Abstract
This study deals with an effective nucleic acids extraction method from various strains of Botryococcus braunii which possesses an extensive extracellular matrix. A method combining freeze/thaw and bead-beating with heterogeneous diameter of silica/zirconia beads was optimized to isolate DNA and RNA from microalgae, especially from B. braunii. Eukaryotic Microalgal Nucleic Acids Extraction (EMNE) method developed in this study showed at least 300 times higher DNA yield in all strains of B. braunii with high integrity and 50 times reduced working volume compared to commercially available DNA extraction kits. High quality RNA was also extracted using this method and more than two times the yield compared to existing methods. Real-time experiments confirmed the quality and quantity of the input DNA and RNA extracted using EMNE method. The method was also applied to other eukaryotic microalgae, such as diatoms, Chlamydomonas sp., Chlorella sp., and Scenedesmus sp. resulting in higher efficiencies. Cost-effectiveness analysis of DNA extraction by various methods revealed that EMNE method was superior to commercial kits and other reported methods by >15%. This method would immensely contribute to area of microalgal genomics.
ISSN
1932-6203
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pone.0037770
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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