Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase = 덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성

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dc.contributor.authorJin-A Ko-
dc.contributor.authorYoung Bae Ryu-
dc.contributor.authorTae Soon Park-
dc.contributor.authorHyung Jae Jung-
dc.contributor.authorJang Hoon Kim-
dc.contributor.authorSu-Jin Park-
dc.contributor.authorJoong Su Kim-
dc.contributor.authorD Kim-
dc.contributor.authorYoung Min Kim-
dc.contributor.authorWoo Song Lee-
dc.date.accessioned2017-04-19T09:30:49Z-
dc.date.available2017-04-19T09:30:49Z-
dc.date.issued2012-
dc.identifier.issn1017-7825-
dc.identifier.uri10.4014/jmb.1202.02007ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10758-
dc.description.abstractPuerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEnzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase = 덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성-
dc.title.alternativeEnzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number9-
dc.citation.endPage1229-
dc.citation.startPage1224-
dc.citation.volume22-
dc.contributor.affiliatedAuthorJin-A Ko-
dc.contributor.affiliatedAuthorYoung Bae Ryu-
dc.contributor.affiliatedAuthorTae Soon Park-
dc.contributor.affiliatedAuthorHyung Jae Jung-
dc.contributor.affiliatedAuthorJang Hoon Kim-
dc.contributor.affiliatedAuthorSu-Jin Park-
dc.contributor.affiliatedAuthorJoong Su Kim-
dc.contributor.affiliatedAuthorYoung Min Kim-
dc.contributor.affiliatedAuthorWoo Song Lee-
dc.contributor.alternativeName고진아-
dc.contributor.alternativeName류영배-
dc.contributor.alternativeName박태순-
dc.contributor.alternativeName정형재-
dc.contributor.alternativeName김장훈-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName김중수-
dc.contributor.alternativeName김도만-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeName이우송-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 22, no. 9, pp. 1224-1229-
dc.identifier.doi10.4014/jmb.1202.02007-
dc.subject.keywordDextransucrase-
dc.subject.keywordLeuconostoc lactis-
dc.subject.keywordPuerarin-
dc.subject.keywordTransglucosylation-
dc.subject.keywordWater solubility-
dc.subject.localDextransucrase-
dc.subject.localLeuconostoc lactis-
dc.subject.localPuerarin-
dc.subject.localTransglucosylation-
dc.subject.localWater solubility-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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