Ribonuclease E modulation of the bacterial SOS response = Ribonuclease E에 의한 박테리아 SOS 감응성의 조절

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Title
Ribonuclease E modulation of the bacterial SOS response = Ribonuclease E에 의한 박테리아 SOS 감응성의 조절
Author(s)
R Manasherob; C Miller; Kwang-Sun Kim; S N Cohen
Bibliographic Citation
PLoS One, vol. 7, no. 6, pp. e38426-e38426
Publication Year
2012
Abstract
Plants, animals, bacteria, and Archaea all have evolved mechanisms to cope with environmental or cellular stress. Bacterial cells respond to the stress of DNA damage by activation of the SOS response, the canonical RecA/LexA-dependent signal transduction pathway that transcriptionally derepresses a multiplicity of genes-leading to transient arrest of cell division and initiation of DNA repair. Here we report the previously unsuspected role of E. coli endoribonuclease RNase E in regulation of the SOS response. We show that RNase E deletion or inactivation of temperature-sensitive RNase E protein precludes normal initiation of SOS. The ability of RNase E to regulate SOS is dynamic, as down regulation of RNase E following DNA damage by mitomycin C resulted in SOS termination and restoration of RNase E function leads to resumption of a previously aborted response. Overexpression of the RraA protein, which binds to the C-terminal region of RNase E and modulates the actions of degradosomes, recapitulated the effects of RNase E deficiency. Possible mechanisms for RNase E effects on SOS are discussed.
ISSN
1932-6203
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pone.0038426
Type
Article
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1. Journal Articles > Journal Articles
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