Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves

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Title
Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves
Author(s)
Yun Hee Kim; Jae Cheol Jeong; Seyeon Park; Haeng Soon Lee; Sang Soo Kwak
Bibliographic Citation
Journal of Plant Physiology, vol. 169, no. 11, pp. 1112-1120
Publication Year
2012
Abstract
Two ethylene response factor (ERF) genes, IbERF1 and IbERF2, were isolated from a library of expressed sequence tags (EST) prepared from suspension-cultured cells and dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). The deduced IbERFs contained a nuclear localization signal and the AP2/ERF DNA-binding domain. RT-PCR analysis revealed that IbERF1 was expressed abundantly during the growth of suspension-cultured cells, whereas the expression levels of IbERF2 transcripts were high in fibrous, thick pigmented roots. Two ERF genes also showed different responses to various types of abiotic stress and pathogen infection. Transient expression of the two ERF genes in tobacco (Nicotiana tabacum) leaves resulted in increased transcript levels of the pathogenesis-related 5 (PR5) gene, the early response to dehydration ten gene (ERD10), the CuZn superoxide dismutase gene (CuZnSOD) and the catalase gene (CAT). It is suggested that the two ERF genes play roles in the stress defense-signaling pathway as transcriptional regulators of the PR5, ERD10, CuZnSOD and CAT genes.
Keyword
Environmental stressEthylene response factorSweetpotatoTobaccoTransient expression assay
ISSN
0176-1617
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jplph.2012.03.002
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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