Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Abstract

A gram-negative, non-flagellated and ovoid- to rod-shaped bacterial strain, designated GSW-M15 T, was isolated from seawater on the southern coast of South Korea. Strain GSW-M15 T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain GSW-M15 T belonged to the genus Roseovarius. Strain GSW-M15 T exhibited highest 16S rRNA gene sequence similarity values (98.3 and 97.5 %) to Roseovarius halotolerans HJ50 T and Roseovarius pacificus 81-2 T and 92.8-96.2 % sequence similarity values to the type strains of the other Roseovarius species. Strain GSW-M15 T contained Q-10 as the predominant ubiquinone and C 18:1 ω7c and 11-methyl-C 18:1 ω7c as the major fatty acids. The major polar lipids detected in strain GSW-M15 Twere phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain GSW-M15 T was 62.9 mol% and its mean DNA-DNA relatedness values with R. halotolerans KCTC 22224 T and R. pacificus LMG 24575 T were 33 and 18 %, respectively. Differential phenotypic properties of strain GSW-M15 T, together with the phylogenetic and genetic distinctiveness, demonstrated that this strain is distinguishable from other Roseovarius species. On the basis of the data presented here, strain GSW-M15 T (=KCTC 23897 T = CCUG 62218 T) represents a novel species of the genus Roseovarius, for which the name Roseovarius litoreus sp. nov. is proposed.