O-GlcNAc regulates pluripotency and reprogramming by directly acting on core components of the pluripotency network

Cited 216 time in scopus
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dc.contributor.authorH Jang-
dc.contributor.authorT W Kim-
dc.contributor.authorS Yoon-
dc.contributor.authorS Y Choi-
dc.contributor.authorT W Kang-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorY W Kwon-
dc.contributor.authorE J Cho-
dc.contributor.authorH D Youn-
dc.date.accessioned2017-04-19T09:32:09Z-
dc.date.available2017-04-19T09:32:09Z-
dc.date.issued2012-
dc.identifier.issn1934-5909-
dc.identifier.uri10.1016/j.stem.2012.03.001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10802-
dc.description.abstractO-linked-N-acetylglucosamine (O-GlcNAc) has emerged as a critical regulator of diverse cellular processes, but its role in embryonic stem cells (ESCs) and pluripotency has not been investigated. Here we show that O-GlcNAcylation directly regulates core components of the pluripotency network. Blocking O-GlcNAcylation disrupts ESC self-renewal and reprogramming of somatic cells to induced pluripotent stem cells. The core reprogramming factors Oct4 and Sox2 are O-GlcNAcylated in ESCs, but the O-GlcNAc modification is rapidly removed upon differentiation. O-GlcNAc modification of threonine 228 in Oct4 regulates Oct4 transcriptional activity and is important for inducing many pluripotency-related genes, including Klf2, Klf5, Nr5a2, Tbx3, and Tcl1. A T228A point mutation that eliminates this O-GlcNAc modification reduces the capacity of Oct4 to maintain ESC self-renewal and reprogram somatic cells. Overall, our study makes a direct connection between O-GlcNAcylation of key regulatory transcription factors and the activity of the pluripotency network.-
dc.publisherElsevier-Cell Press-
dc.titleO-GlcNAc regulates pluripotency and reprogramming by directly acting on core components of the pluripotency network-
dc.title.alternativeO-GlcNAc regulates pluripotency and reprogramming by directly acting on core components of the pluripotency network-
dc.typeArticle-
dc.citation.titleCell Stem Cell-
dc.citation.number7-
dc.citation.endPage74-
dc.citation.startPage62-
dc.citation.volume11-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.alternativeName장현철-
dc.contributor.alternativeName김태완-
dc.contributor.alternativeName윤성호-
dc.contributor.alternativeName최수연-
dc.contributor.alternativeName강태욱-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName권유욱-
dc.contributor.alternativeName조은정-
dc.contributor.alternativeName윤홍덕-
dc.identifier.bibliographicCitationCell Stem Cell, vol. 11, no. 7, pp. 62-74-
dc.identifier.doi10.1016/j.stem.2012.03.001-
dc.description.journalClassY-
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