Increasing γ-linolenic acid content in Spirulina platensis using fatty acid supplement and light-dark illumination

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Title
Increasing γ-linolenic acid content in Spirulina platensis using fatty acid supplement and light-dark illumination
Author(s)
E K Kim; G G Choi; Hee-Sik KimChi-Yong AhnHee-Mock Oh
Bibliographic Citation
Journal of Applied Phycology, vol. 24, no. 4, pp. 743-750
Publication Year
2012
Abstract
Spirulina platensis, a filamentous cyanobacterium, produces γ-linolenic acid (GLA, 18:3), which is an important anti-inflammatory for pharmaceutical use. Thus, to increase the GLA content in S. platensis, this study investigated the combined effect of a light-dark (LD) two-stage culture and mixotrophic culture including a precursor of GLA. When compared with a photoautotrophic culture, the supplement of a GLA precursor, such as a long- or short-chain carbon source, enhanced the total fatty acid and GLA contents in the cells in the two-stage culture. The highest GLA content of 2% (w/w) and productivity of 27. 6 ± 4. 7 mg L -1 were obtained in S. platensis when using 0. 01 mM palmitic acid as a supplement in the two-stage culture. This study also suggests that a mixotrophic and LD two-stage culture may represent a method for increasing the total lipid production, which can then be converted to biofuels.
Keyword
γ-Linolenic acid (GLA)Fatty acidsMixotrophSpirulina platensisTwo-stage culture
ISSN
0921-8971
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10811-011-9693-0
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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