Cited 7 time in
- A rapid and simple method for preparing an insoluble substrate for screening of microbial xylanase = 신속하고 간단한 자일라네이스 탐색 방법
- Kyong-Cheol Ko; Yunjon Han; Bong Seok Shin; Jong Hyun Choi; Jae Jun Song
- Bibliographic Citation
- Applied Biochemistry and Biotechnology, vol. 167, no. 4, pp. 677-684
- Publication Year
- Several types of enzymes, including cellulases and xylanases, are required to degrade hemicelluloses and cellulose, which are major components of lignocellulosic biomass. Such degradative processes can be used to produce various useful industrial biomaterials. Screening methods for detecting polysaccharide-degrading microorganisms include the use of dye-labeled substrates in growth medium and culture plate staining techniques. However, the preparation of screening plates, which typically involves chemical cross-linking to synthesize a dye-labeled substrate, is a complicated and time-consuming process. Moreover, such commercial substrates are very expensive, costing tenfold more than the natural xylan. Staining methods are also problematic because they may damage relevant microorganisms and are associated with contamination of colonies of desirable organisms with adjacent unwanted bacteria. In the present study, we describe a sonication method for the simple and rapid preparation of an insoluble substrate that can be used to screen for xylanase-expressing bacteria in microbial populations. Using this new method, we have successfully isolated a novel xylanase gene from a xylolytic microorganism termed Xyl02-KBRB and Xyl14-KBRB in the bovine rumen.
- Assay method; Dye substrate; Screening method; Xylanase
- Appears in Collections:
- Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.