A rapid and simple method for preparing an insoluble substrate for screening of microbial xylanase = 신속하고 간단한 자일라네이스 탐색 방법

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Title
A rapid and simple method for preparing an insoluble substrate for screening of microbial xylanase = 신속하고 간단한 자일라네이스 탐색 방법
Author(s)
Kyong-Cheol KoYunjon Han; Bong Seok Shin; Jong Hyun ChoiJae Jun Song
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 167, no. 4, pp. 677-684
Publication Year
2012
Abstract
Several types of enzymes, including cellulases and xylanases, are required to degrade hemicelluloses and cellulose, which are major components of lignocellulosic biomass. Such degradative processes can be used to produce various useful industrial biomaterials. Screening methods for detecting polysaccharide-degrading microorganisms include the use of dye-labeled substrates in growth medium and culture plate staining techniques. However, the preparation of screening plates, which typically involves chemical cross-linking to synthesize a dye-labeled substrate, is a complicated and time-consuming process. Moreover, such commercial substrates are very expensive, costing tenfold more than the natural xylan. Staining methods are also problematic because they may damage relevant microorganisms and are associated with contamination of colonies of desirable organisms with adjacent unwanted bacteria. In the present study, we describe a sonication method for the simple and rapid preparation of an insoluble substrate that can be used to screen for xylanase-expressing bacteria in microbial populations. Using this new method, we have successfully isolated a novel xylanase gene from a xylolytic microorganism termed Xyl02-KBRB and Xyl14-KBRB in the bovine rumen.
Keyword
Assay methodDye substrateScreening methodXylanase
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12010-012-9722-8
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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