Soluble expression of OmpA from Haemophilus parasuis in Escherichia coli and its protective effects in the mouse model of infection

Cited 4 time in scopus
Metadata Downloads
Title
Soluble expression of OmpA from Haemophilus parasuis in Escherichia coli and its protective effects in the mouse model of infection
Author(s)
Jungoh Ahn; M Hong; S Yoo; Eun Gyo Lee; H Won; I Yoon; Joon Ki Jung; Hong-Weon Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 22, no. 9, pp. 1307-1309
Publication Year
2012
Abstract
Haemophilus parasuis causes contagious porcine Glasser's disease leading to severe losses in the swine industry. In this study, we established an efficient Escherichia colibased system for the expression of H. parasuis major outer-membrane protein (MOMP) that has been known as a good vaccine candidate against Glasser's disease. Use of an E. coli-derived pelB leader sequence made it possible to produce recombinant MOMP (rMOMP) as the soluble forms without an additional refolding process. Using two different animal models, it was evaluated that the rMOMP was capable of inducing a significant immune response and providing protection against H. parasuis infection.
Keyword
AntigenEscherichia coliHaemophilus parasuisOmpApelB leader sequenceSoluble expression
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1201.01003
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.