Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity

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Title
Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity
Author(s)
E Y Kim; Won Kon Kim; Hyo Jin Kang; Jeong Hoon Kim; Sang Jeon Chung; Y S Seo; Sung Goo ParkSang Chul LeeKwang-Hee Bae
Bibliographic Citation
Journal of Lipid Research, vol. 53, no. 9, pp. 1864-1876
Publication Year
2012
Abstract
Acetylation is one of the most crucial posttranslational modifications that affect protein function. Protein lysine acetylation is catalyzed by acetyltransferases, and acetyl-CoA functions as the source of the acetyl group. Additionally, acetyl-CoA plays critical roles in maintaining the balance between carbohydrate metabolism and fatty acid synthesis. Here, we sought to determine whether lysine acetylation is an important process for adipocyte differentiation. Based on an analysis of the acetylome during adipogenesis, various proteins displaying significant quantitative changes were identified by LC-MS/MS. Of these identified proteins, we focused on malate dehydrogenase 1 (MDH1). The acetylation level of MDH1 was increased up to 6-fold at the late stage of adipogenesis. Moreover, overexpression of MDH1 in 3T3-L1 preadipocytes induced a significant increase in the number of cells undergoing adipogenesis. The introduction of mutations to putative lysine acetylation sites showed a significant loss of the ability of cells to undergo adipogenic differentiation. Furthermore, the acetylation of MDH1 dramatically enhanced its enzymatic activity and subsequently increased the intracellular levels of NADPH. These results clearly suggest that adipogenic differentiation may be regulated by the acetylation of MDH1 and that the acetylation of MDH1 is one of the cross-talk mechanisms between adipogenesis and the intracellular energy level.
Keyword
Acetyl-CoAAdipogenesisObesityProtein acetylation
ISSN
0022-2275
Publisher
Amer Soc Biochemistry Molecular Biology Inc
DOI
http://dx.doi.org/10.1194/jlr.M026567
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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