Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)

Cited 8 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMinhwa Do-
dc.contributor.authorWon-Gu Jang-
dc.contributor.authorJ H Hwang-
dc.contributor.authorHoon Jang-
dc.contributor.authorEun Jung Kim-
dc.contributor.authorEun Jeong Jeong-
dc.contributor.authorH Shim-
dc.contributor.authorS S Hwang-
dc.contributor.authorK B Oh-
dc.contributor.authorS J Byun-
dc.contributor.authorJ H Kim-
dc.contributor.authorJeong Woong Lee-
dc.date.accessioned2017-04-19T09:32:53Z-
dc.date.available2017-04-19T09:32:53Z-
dc.date.issued2012-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2012.07.031ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10856-
dc.description.abstractSomatic cell nuclear transfer (SCNT) has been established for the transmission of specific nuclear DNA. However, the fate of donor mitochondrial DNA (mtDNA) remains unclear. Here, we examined the fate of donor mtDNA in recloned pigs through third generations. Fibroblasts of recloned pigs were obtained from offspring of each generation produced by fusion of cultured fibroblasts from a Minnesota miniature pig (MMP) into enucleated oocytes of a Landrace pig. The D-loop regions from the mtDNA of donor and recipient differ at nucleotide sequence positions 16050 (A→T), 16062 (T→C), and 16135 (G→A). In order to determine the fate of donor mtDNA in recloned pigs, we analyzed the D-loop region of the donor's mtDNA by allele-specific PCR (AS-PCR) and real-time PCR. Donor mtDNA was successfully detected in all recloned offspring (F1, F2, and F3). These results indicate that heteroplasmy that originate from donor and recipient mtDNA is maintained in recloned pigs, resulting from SCNT, unlike natural reproduction.-
dc.publisherElsevier-
dc.titleInheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)-
dc.title.alternativeInheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number3-
dc.citation.endPage770-
dc.citation.startPage765-
dc.citation.volume424-
dc.contributor.affiliatedAuthorMinhwa Do-
dc.contributor.affiliatedAuthorWon-Gu Jang-
dc.contributor.affiliatedAuthorHoon Jang-
dc.contributor.affiliatedAuthorEun Jung Kim-
dc.contributor.affiliatedAuthorEun Jeong Jeong-
dc.contributor.affiliatedAuthorJeong Woong Lee-
dc.contributor.alternativeName도민화-
dc.contributor.alternativeName장원구-
dc.contributor.alternativeName황정희-
dc.contributor.alternativeName장훈-
dc.contributor.alternativeName김은정-
dc.contributor.alternativeName정은정-
dc.contributor.alternativeName심호섭-
dc.contributor.alternativeName황성수-
dc.contributor.alternativeName오건봉-
dc.contributor.alternativeName변성준-
dc.contributor.alternativeName김진회-
dc.contributor.alternativeName이정웅-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 424, no. 3, pp. 765-770-
dc.identifier.doi10.1016/j.bbrc.2012.07.031-
dc.subject.keywordMitochondrial DNA-
dc.subject.keywordRecloned pig-
dc.subject.keywordSomatic cell nuclear transfer-
dc.subject.localmitochondrial DNA-
dc.subject.localMitochondrial DNA-
dc.subject.localRecloned pig-
dc.subject.localsomatic cell nuclear transfer-
dc.subject.localSomatic cell nuclear transfer-
dc.subject.localSomatic cell nuclear transfer (SCNT)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.