Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions = 덱스트란나아제 및 환상올리고당 합성반응을 촉매하는 Bacteroides thetaiotaomicron VPI-5482 유래 효소

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dc.contributor.authorYoung Min Kim-
dc.contributor.authorE Yamamoto-
dc.contributor.authorM S Kang-
dc.contributor.authorH Nakai-
dc.contributor.authorW Saburi-
dc.contributor.authorM Okuyama-
dc.contributor.authorH Mori-
dc.contributor.authorK Funane-
dc.contributor.authorM Momma-
dc.contributor.authorZ Fujimoto-
dc.contributor.authorM Kobayashi-
dc.contributor.authorD Kim-
dc.contributor.authorA Kimura-
dc.date.accessioned2017-04-19T09:33:00Z-
dc.date.available2017-04-19T09:33:00Z-
dc.date.issued2012-
dc.identifier.issn1742464X-
dc.identifier.uri10.1111/j.1742-4658.2012.08698.xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10860-
dc.description.abstractBacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.-
dc.publisherWiley-
dc.titleBacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions = 덱스트란나아제 및 환상올리고당 합성반응을 촉매하는 Bacteroides thetaiotaomicron VPI-5482 유래 효소-
dc.title.alternativeBacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions-
dc.typeArticle-
dc.citation.titleFEBS Journal-
dc.citation.number0-
dc.citation.endPage3191-
dc.citation.startPage3185-
dc.citation.volume279-
dc.contributor.alternativeName김영민-
dc.contributor.alternativeNameYamamoto-
dc.contributor.alternativeName강민선-
dc.contributor.alternativeNameNakai-
dc.contributor.alternativeNameSaburi-
dc.contributor.alternativeNameOkuyama-
dc.contributor.alternativeNameMori-
dc.contributor.alternativeNameFunane-
dc.contributor.alternativeNameMomma-
dc.contributor.alternativeNameFujimoto-
dc.contributor.alternativeNameKobayashi-
dc.contributor.alternativeName김도만-
dc.contributor.alternativeNameKimura-
dc.identifier.bibliographicCitationFEBS Journal, vol. 279, pp. 3185-3191-
dc.identifier.doi10.1111/j.1742-4658.2012.08698.x-
dc.subject.keywordBacteroides thetaiotaomicron VPI-5482-
dc.subject.keywordcycloisomaltooligosaccharide glucanotransferase-
dc.subject.keywordendo-dextranase-
dc.subject.keywordglycoside hydrolase family 66-
dc.subject.keywordisomaltotetraose-
dc.subject.localBacteroides thetaiotaomicron VPI-5482-
dc.subject.localcycloisomaltooligosaccharide glucanotransferase-
dc.subject.localCycloisomaltooligosaccharide glucanotransferase-
dc.subject.localendo-dextranase-
dc.subject.localEndo-dextranase-
dc.subject.localglycoside hydrolase family 66-
dc.subject.localGlycoside hydrolase family 66-
dc.subject.localisomaltotetraose-
dc.description.journalClassY-
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