DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sang Jick Kim | - |
dc.contributor.author | H J Hong | - |
dc.date.accessioned | 2017-04-19T09:33:00Z | - |
dc.date.available | 2017-04-19T09:33:00Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | 10.1007/978-1-61779-974-7-14 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/10867 | - |
dc.description.abstract | Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world's first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody. | - |
dc.publisher | Springer | - |
dc.title | Humanization by guided selections | - |
dc.title.alternative | Humanization by guided selections | - |
dc.type | Article | - |
dc.citation.title | Methods in Molecular Biology | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 257 | - |
dc.citation.startPage | 247 | - |
dc.citation.volume | 907 | - |
dc.contributor.affiliatedAuthor | Sang Jick Kim | - |
dc.contributor.alternativeName | 김상직 | - |
dc.contributor.alternativeName | 홍효정 | - |
dc.identifier.bibliographicCitation | Methods in Molecular Biology, vol. 907, pp. 247-257 | - |
dc.identifier.doi | 10.1007/978-1-61779-974-7-14 | - |
dc.subject.keyword | Antibody library | - |
dc.subject.keyword | Chain shuffling | - |
dc.subject.keyword | Guided selection | - |
dc.subject.keyword | Human antibody | - |
dc.subject.keyword | Panning | - |
dc.subject.keyword | Phage display | - |
dc.subject.local | Antibody library | - |
dc.subject.local | antibody library | - |
dc.subject.local | Chain shuffling | - |
dc.subject.local | chain shuffling | - |
dc.subject.local | Guided selection | - |
dc.subject.local | guided selection | - |
dc.subject.local | Human antibody | - |
dc.subject.local | human antibody | - |
dc.subject.local | panning | - |
dc.subject.local | Panning | - |
dc.subject.local | Phage display | - |
dc.subject.local | phage display | - |
dc.description.journalClass | N | - |
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