Dynamics of Setdb1 expression in early mouse development

Cited 18 time in scopus
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Title
Dynamics of Setdb1 expression in early mouse development
Author(s)
Sunwha Cho; Jung Sun Park; Sujin Kwon; Yong-Kook Kang
Bibliographic Citation
Gene Expression Patterns, vol. 12, no. 5, pp. 213-218
Publication Year
2012
Abstract
Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expressionwas markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4.
Keyword
Blastocyst outgrowthEmbryonic stem cellHistone methylationMouse embryoSetdb1/Eset
ISSN
1567-133X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.gep.2012.03.005
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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