Activation of Nod1 and Nod2 induces innate immune responses of prostate epithelial cells

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dc.contributor.authorM J Kang-
dc.contributor.authorS K Heo-
dc.contributor.authorE J Song-
dc.contributor.authorD J Kim-
dc.contributor.authorS Y Han-
dc.contributor.authorJ H Han-
dc.contributor.authorBo Yeon Kim-
dc.contributor.authorJ H Park-
dc.date.accessioned2017-04-19T09:33:01Z-
dc.date.available2017-04-19T09:33:01Z-
dc.date.issued2012-
dc.identifier.issn02704137-
dc.identifier.uri10.1002/pros.22483ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10870-
dc.description.abstractBACKGROUND Nod1 and Nod2 are cytosolic receptors which are responsible for sensing bacterial peptidoglycan derivatives. In this study, we determined whether Nod1 and Nod2 are involved in the innate immune responses of prostate epithelial cells. METHODS The expression of Nod1 and Nod2 was examined by RT-PCR and immunohistochemistry. ELISA was performed to determine the production of cytokines/chemokines. Activation of NF-κB and MAPK was examined using western blot analysis. RESULTS The Nod1 gene was distinctly expressed in all tested cells including DU145, PC3, and TRAMP-C2 cells, whereas Nod2 expression was weak. Both Nod1 and Nod2 proteins were expressed in normal mouse prostate epithelia with difference of expression levels. Tri-DAP (Nod1 agonist), but not MDP (Nod2), increased the production of IL-8 (or KC) and IL-6 in prostate epithelial cells. Tri-DAP and MDP could upregulate the gene expression of COX-2 and activate NF-κB and MAPK. In addition, Tri-DAP and MDP synergized with TLR agonists to induce the production of IL-8/KC or IL-6 in PC3 and TRAMP-C2 cells. We finally showed that Nod1 and Nod2 were also expressed in a wide range of prostate lesions including prostate intraepithelial neoplasm (PIN), phyllodes-like tumor, and adenocarcinoma in TRAMP (transgenic adenocarcinoma of the mouse prostate) mice, even though the expression level of Nod1 and Nod2 was different. CONCLUSION These results indicate that Nod1 and Nod2 may play important roles in the innate immune response of prostate epithelial cells and the development and progression of prostate cancer.-
dc.publisherWiley-
dc.titleActivation of Nod1 and Nod2 induces innate immune responses of prostate epithelial cells-
dc.title.alternativeActivation of Nod1 and Nod2 induces innate immune responses of prostate epithelial cells-
dc.typeArticle-
dc.citation.titleProstate-
dc.citation.number12-
dc.citation.endPage1358-
dc.citation.startPage1351-
dc.citation.volume72-
dc.contributor.affiliatedAuthorBo Yeon Kim-
dc.contributor.alternativeName강민정-
dc.contributor.alternativeName허숙경-
dc.contributor.alternativeName송은정-
dc.contributor.alternativeName김동재-
dc.contributor.alternativeName한승윤-
dc.contributor.alternativeName한주희-
dc.contributor.alternativeName김보연-
dc.contributor.alternativeName박종환-
dc.identifier.bibliographicCitationProstate, vol. 72, no. 12, pp. 1351-1358-
dc.identifier.doi10.1002/pros.22483-
dc.subject.keywordcyclooxygenase-2-
dc.subject.keywordinnate immune response-
dc.subject.keywordNod1-
dc.subject.keywordNod2-
dc.subject.keywordprostate epithelial cells-
dc.subject.localcyclooxygenase-2-
dc.subject.localCyclooxygenase 2-
dc.subject.localinnate immune response-
dc.subject.localInnate immune response-
dc.subject.localNod1-
dc.subject.localNod2-
dc.subject.localNOD2-
dc.subject.localprostate epithelial cells-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
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