Violaceols function as actin inhibitors inducing cell shape elongation in fibroblast cells

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Title
Violaceols function as actin inhibitors inducing cell shape elongation in fibroblast cells
Author(s)
Y Asami; Jae-Hyuk Jang; H Oh; J H Sohn; Jong Won Kim; D O Moon; Osong Kwon; M Kawatani; H Osada; Bo Yeon KimJong Seog Ahn
Bibliographic Citation
Bioscience Biotechnology and Biochemistry, vol. 76, no. 8, pp. 1431-1437
Publication Year
2012
Abstract
Violaceol-I and -II were isolated from a fractionated library of marine-derived fungal metabolites. These compounds increased the calcium ion concentration inside the cell and caused F-actin aggregation in rat fibroblast 3Y1 cells within 3 h resulting in cell shape elongation. Calcium chelator BAPTA-AM (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester) inhibited violaceol-I and -II induced F-actin aggregation in 3Y1 cells, and hence violaceol-I and -II act in a calcium dependent manner. Violaceol-I and -II inhibited G-actin polymerization in vitro in a dose-dependent manner and strongly associated with G-actin, at dissociation equilibrium constants of 1:44 × 10 -8 M and 2:52 × 10 -9 M respectively. Here we report the identification of a novel function of violaceol-I and -II as actin inhibitors. Violaceol-I and -II induced cell shape elongation through F-actin aggregation in 3Y1 fibroblasts. These compounds may give researchers new insights into the role of actin in tumorigenesis and lead to the development of additional anti-tumor drugs.
Keyword
ActinCalcium ionCell shape elongationFractionated libraryViolaceols
ISSN
0916-8451
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1271/bbb.120074
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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