Somatic embryogenesis in leaf tissue culture of Soapberry (Sapindus mukorossi Gaertn.)

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Title
Somatic embryogenesis in leaf tissue culture of Soapberry (Sapindus mukorossi Gaertn.)
Author(s)
Hyun Tae Kim; B H Yang; Y G Park; Jang Ryol Liu
Bibliographic Citation
Plant Biotechnology, vol. 29, no. 3, pp. 311-314
Publication Year
2012
Abstract
Leaf explants formed embryogenic calluses at a frequency of 53.9% when cultured on B5 media supplemented with 0.1 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.01 mg l -1 6-benzyladenine (BA) for 6 weeks. Upon transfer onto media with 5 mg l -1abscisic acid, embryogenic calluses yielded somatic embryos at 73%. Somatic embryos developed into plantlets on media without plant growth regulators at 90%. Embryogenic calluses proliferated and maintained embryogenic capacity when subcultured on media with 0.1 mg l -1 2,4-D and 0.01 mg l -1 BA at 4-week intervals. This culture system is an effective means for clonal propagation and genetic manipulation of soapberry because it ensures taproot development required for tree stability.
Keyword
Embryogenic callusPlant regenerationSapindus mukorossiSomatic embryo
ISSN
1342-4580
Publisher
Japanese Soc Plant Cell & Molecular Biology
DOI
http://dx.doi.org/10.5511/plantbiotechnology.12.0330a
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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