Structures of the gamma-class carbonic anhydrase homologue YrdA suggest a possible allosteric switch

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Title
Structures of the gamma-class carbonic anhydrase homologue YrdA suggest a possible allosteric switch
Author(s)
H M Park; J H Park; J W Choi; J Lee; Bo Yeon Kim; C H Jung; J S Kim
Bibliographic Citation
Acta Crystallographica Section D-Biological Crystallography, vol. 68, no. 8, pp. 920-926
Publication Year
2012
Abstract
The YrdA protein shows high sequence similarity to -class carbonic anhydrase (-CA) proteins and is classified as part of the -CA protein family. However, its function has not been fully elucidated as it lacks several of the conserved residues that are considered to be necessary for -CA catalysis. Interestingly, a homologue of -CA from Methanosarcina thermophila and a Β-carboxysomal -CA from a Β-cyano-bacterium have shown that these catalytic residues are not always conserved in -CAs. The crystal structure of YrdA from Escherichia coli (ecYrdA) is reported here in two crystallographic forms. The overall structure of ecYrdA is also similar to those of the -CAs. One loop around the putative catalytic site shows a number of alternative conformations. A His residue (His70) on this loop coordinates with, or is reoriented from, the catalytic Zn 2+ ion; this is similar to the conformations mediated by an Asp residue on the catalytic loops of Β-CA proteins. One Trp residue (Trp171) also adopts two alternative conformations that may be related to the spatial positions of the catalytic loop. Even though significant CA activity could not be detected using purified ecYrdA, these structural features have potential functional implications for -CA-related proteins
Keyword
class carbonic anhydrasesYrdA
ISSN
0907-4449
Publisher
Int Union Crystallography
DOI
http://dx.doi.org/10.1107/S0907444912017210
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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