An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy

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Title
An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy
Author(s)
Y Her; Y C Lee; J H Oh; Y E Choi; Chang Woo Lee; J S Kim; H M Kim; J W Yang
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 17, no. 3, pp. 538-546
Publication Year
2012
Abstract
Over the past several decades, the pharmacological effects of ginsenosides in Panax ginseng roots have been extensively investigated. Here, we developed a method for producing specific ginsenosides (F1 and F2) with good yields (F1:162 mg/g, F2:305 mg/g) using β-glycosidase purified from Aspergillus niger. In addition, each ginsenoside (at least 25 species) was separated and purified by high performance liquid chromatography (HPLC) using five different types of solvents and different purification steps. In addition, the Rg3:Rh2 mixture (1:1, w/w) was shown to inhibit a specific lung cancer cell line (NCI-H232) in vivo, displaying an anticancer effect at a dose lower than achieved using treatments with single Rg3 or Rh2. This finding suggests that the combination of ginsenosides for targeting anticancer is more effective than the use of a single ginsenoside from ginseng or red ginseng.
Keyword
Enzymatic processGinsenoside combinationGinsenosidesLung cancer cell line (NCI-H23)PurificationSolvent system
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/s12257-011-0678-2
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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