Celeribacter baekdonensis sp. nov., isolated from seawater, and emended description of the genus Celeribacter Ivanova et al. 2010

Abstract

A Gram-stain-negative, non-motile, ovoid or rod-shaped bacterial strain, designated L-6 T, was isolated from seawater of Baekdo harbour of the East Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain L-6 T grew optimally at 30 °C, at pH 7.5-8.0 and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain L-6 T formed a cluster with the type strain of Celeribacter neptunius at a bootstrap resampling value of 100 %. Strain L-6 T exhibited 16S rRNA gene sequence similarity values of 97.7 % to C. neptunius H 14 T and of less than 96.2 % to the type strains of other species used in the phylogenetic analysis. The G+C content of the chromosomal DNA of strain L-6 T was 60.9 mol%. The predominant ubiquinone found in strain L-6 T and C. neptunius CIP 109922 Twas ubiquinone-10 (Q-10). The predominant fatty acid of strain L-6 T and C. neptunius CIP 109922 T was C 18: 1ω7c. The major polar lipids of strain L-6 T were phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The mean level of DNA-DNA relatedness between strain L-6 T and C. neptunius CIP 109922 T was 17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain L-6 T is distinguishable from C. neptunius. On the basis of the data presented, strain L-6 T is considered to represent a novel species of the genus Celeribacter, for which the name Celeribacter baekdonensis sp. nov. is proposed. The type strain is L-6 T (= KCTC 23497 T = CCUG 60799 T).